The influence of chitosan and quaternized chitosan on Pseudomonas aeruginosa biofilm formation

›› 2012, Vol. 27 ›› Issue (10): 854-857.

The influence of chitosan and quaternized chitosan on Pseudomonas aeruginosa biofilm formation

1. Departemtn of Clinical Laboratory，Shanghai Mental Health Center，Shanghai Jiaotong University School of Medicine，Shanghai 200030，China； 2. Faculty of Medical Laboratory Science，Ruijin Hospital，Shanghai Jiaotong University School of Medicine，Shanghai 200025，China； 3. Laboratory of Pathogenic Biology，Shanghai Jiaotong University School of Medicine，Shanghai 200025，China； 4. Shanghai Nineth People's Hospital，Shanghai Jiaotong University School of Medicine，Shanghai 200011，China；5. Laboratory of Medical Science Institute，Shanghai Tongji Hospital，Shanghai 200065，China

Received:2012-09-04 Revised:2012-09-14 Online:2012-10-30 Published:2012-10-12
Contact: ping LIN

Abstract

Abstract: Objective　To investigate the broad-spectrum antibacterial efficacy of chitosan (CHI) and its derivatives. Methods　The antibacterial test of Pseudomonas aeruginosa was performed by CHI and quaternized chitosan (QCHI). The volumes of per 1 mL at 0，3，6，9 and 12 h by CHI and QCHI were determined. The results were compared with those of control hole volume. The Pseudomonas aeruginosa RNA was extracted simultaneously. The ahpF，pa3187，aprA and rpoS genes were amplified，and their expressions were determined. The remaining Pseudomonas aeruginosa were cultured by LB medium for 3 d. The floating bacteria were flushed by PBS buffer solution，and were stained by crystallization violet. After decolorizing by 95% ethanol，the biofilm formation function of Pseudomonas aeruginosa was analyzed by enzyme-labeled analyzer at 570 nm. Results　There were significant differences between CHI and QCHI hole volumes of live bacteria after culturing 6 h compared with control hole volumes，but there was no obvious difference CHI and QCHI volumes. The pa3187 expression was increased by CHI and QCHI，but aprA expression was decreased by them. QCHI can decrease ahpF expression，but CHI can improve ahpF expression. The rpoS expression was also decreased by QCHI. Biofilm formation showed that the significant inhibitory effect could be seen in Pseudomonas aeruginosa biofilm formation by CHI and QCHI from culturing 6 h. Conclusions　In a certain extent，CHI and QCHI can inhibit the proliferation and biofilm formation of Pseudomonas aeruginosa.

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Key words: Chitosan, Quaternized chitosan, Pseudomonas aeruginosa, Antibacterial test, Biofilm formation

LIN Ping 1，ZHAO Yufan 2，WU Yue 2，ZHANG Xiao 2，GUO Xiaokui 3，TAN Honglue 4，LI Qingtian 2，SUN Yang 5. The influence of chitosan and quaternized chitosan on Pseudomonas aeruginosa biofilm formation[J]. , 2012, 27(10): 854-857.

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