Abstract: Objective　To analyze and evaluate the clinical application of tumor marker detection kit ［microarray enzymelinked immunosorbent assay(ArrayELISA)］ in determining 6 tumor markers.  Methods　Electrochemiluminescence immunoassay(ECLIA) analyzer was used to detect 6 clinically common tumor markers: alpha fetoprotein (AFP), carcinoembryonic antigen （CEA）, prostate specific antigen（PSA）, carbohydrate antigen 125（CA125）, carbohydrate antigen 199（CA199） and carbohydrate antigen 153（CA153）. The receiver operating characteristic（ROC）curve was evaluated.  Results　There were no significant differences in the positive rates of AFP, CEA, PSA and CA125 by the 2 methods（P>0.05），while the positive rates of CA199 and CA153 had statistical significance (P<0.01). The determination results of AFP, CEA, PSA, CA125 and CA199 by the 2 methods showed high total coincidence rate (>85%)，and the total coincidence rate of CA153 was   61.1%. The correlation was significant ［P<0.01, correlation coefficient (r)=0.6950.960］, and areas under ROC curves of AFP, CEA, PSA, CA125 and CA199 were 0.620.99. The area under ROC curve of CA153 by ArrayELISA was lower than that of ECLIA in the diagnosis of breast carcinoma.  Conclusions　Both ArrayELISA and ECLIA show good coincidence in detecting the 6 tumor markers, and ArrayELISA could be introduced for clinical routine examination and screening testing of determining 6 tumor markers. 

Key words: Microarray enzymelinked immunosorbent assay, Electrochemiluminescence immunoassay, Methodology comparison, Tumor marker