Roles of serum TNF-α，hs-CRP and IL-22 in assessing disease activity and prognosis in children with Crohn's disease

doi:10.3969/j.issn.1673-8640.2026.04.012

Abstract

Abstract:

Objective To investigate the relationship between serum tumor necrosis factor-alpha（TNF-α），high-sensitivity C-reactive protein（hs-CRP）and interleukin-22（IL-22）and the disease activity and prognosis of pediatric Crohn's disease（CD）. Methods Totally，63 children with CD（CD group）and 63 healthy children（healthy control group） were enrolled from January 2017 to June 2022 at the Children's Hospital of Zhengzhou University. The levels of serum TNF-α，hs-CRP and IL-22 were determined. All the children were classified into non-active group（26 cases）and active group（37 cases）based on the pediatric Crohn's disease activity index（PCDAI）score. All the children were followed up for 1 year，and they were classified into poor prognosis group（24 cases）and good prognosis group（39 cases）based on the prognosis. Pearson correlation analysis was used to evaluate the correlation between the levels of serum TNF-α，hs-CRP and IL-22 and the PCDAI score in children with CD. Receiver operating characteristic（ROC）curve was used to evaluate the efficacy of serum TNF-α，hs-CRP and IL-22 in predicting poor prognosis in children with CD. Results The levels of serum TNF-α，hs-CRP and IL-22 in CD group were higher than those in healthy control group（P<0.05）. The PCDAI score and the levels of serum TNF-α，hs-CRP and IL-22 in active group were higher than those in non-active group（P<0.05）. The PCDAI score in children with CD was positively correlated with the levels of serum TNF-α，hs-CRP and IL-22（r values were 0.595，0.656 and 0.643，respectively，P<0.001）. The levels of serum TNF-α，hs-CRP and IL-22 in poor prognosis group were higher than those in good prognosis group （P<0.001）. Elevated levels of serum TNF-α，hs-CRP and IL-22 were risk factors for poor prognosis in children with CD（P<0.05）. The areas under curves（AUC）of serum TNF-α，hs-CRP，IL-22 single and combined determinations in predicting poor prognosis in children with CD were 0.686，0.752，0.682 and 0.848，respectively. Conclusions The levels of serum TNF-α，hs-CRP and IL-22 are related to the disease activity and prognosis of pediatric CD and may be used as the indicators for assessing the disease activity and prognosis of pediatric CD.

Key words: Tumor necrosis factor-alpha, High-sensitivity C-reactive protein, Interleukin-22, Crohn's disease, Disease activity, Prognosis, Children

CLC Number:

R446.1

WANG Xianghui, LI Xiaoqin, XUE Fumin. Roles of serum TNF-α，hs-CRP and IL-22 in assessing disease activity and prognosis in children with Crohn's disease[J]. Laboratory Medicine, 2026, 41(4): 386-390.

Figures/Tables 7

References 18

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组别	例数	TNF-α/ （pg·mL-1）	hs-CRP/ （mg·L-1）	IL-22/ （pg·mL-1）
正常对照组	63	4.91±1.15	6.61±1.85	7.53±1.88
CD组	63	10.54±3.28	12.89±3.10	14.01±2.87
t值		12.857	13.808	14.991
P值		<0.001	<0.001	<0.001

组别	例数	TNF-α/ （pg·mL-1）	hs-CRP/ （mg·L-1）	IL-22/ （pg·mL-1）
正常对照组	63	4.91±1.15	6.61±1.85	7.53±1.88
CD组	63	10.54±3.28	12.89±3.10	14.01±2.87
t值		12.857	13.808	14.991
P值		<0.001	<0.001	<0.001

组别	例数	PCDAI评分/分	TNF-α/（pg·mL-1）	hs-CRP/（mg·L-1）	IL-22/（pg·mL-1）
活动组	37	24.99±8.23	12.00±2.93	14.31±2.41	15.01±2.53
非活动组	26	5.60±3.23	8.47±2.60	10.87±2.87	12.58±2.75
t值		11.391	4.927	5.154	3.621
P值		<0.001	<0.001	<0.001	0.001

组别	例数	PCDAI评分/分	TNF-α/（pg·mL-1）	hs-CRP/（mg·L-1）	IL-22/（pg·mL-1）
活动组	37	24.99±8.23	12.00±2.93	14.31±2.41	15.01±2.53
非活动组	26	5.60±3.23	8.47±2.60	10.87±2.87	12.58±2.75
t值		11.391	4.927	5.154	3.621
P值		<0.001	<0.001	<0.001	0.001

组别	例数	性别		年龄/岁	治疗药物		TNF-α/ （pg·mL-1）	hs-CRP/ （mg·L-1）	IL-22/ （pg·mL-1）
组别	例数	男/例	女/例	年龄/岁	免疫抑制剂/例	生物制剂/例	TNF-α/ （pg·mL-1）	hs-CRP/ （mg·L-1）	IL-22/ （pg·mL-1）
预后不良组	24	15	9	8.92±1.74	12	6	12.02±3.33	14.58±2.07	15.33±2.40
预后良好组	39	20	19	8.26±2.01	16	7	9.63±2.93	11.85±3.18	13.45±2.78
统计值		0.757		1.330	0.485	0.451	2.984	3.740	2.740
P值		0.384		0.188	0.486	0.502	0.004	<0.001	<0.001