Influence of serum HMGB1 and IL-6 levels on pregnancy outcomes of pregnant women with periodontal disease

doi:10.3969/j.issn.1673-8640.2025.10.009

Abstract

Abstract:

Objective To investigate the influence of serum high mobility group protein B1（HMGB1） and interleukin-6（IL-6） levels on the pregnancy outcomes of pregnant women with periodontal disease. Methods From February 2022 to February 2024，200 pregnant women with periodontal disease（periodontal disease group） and 100 pregnant women with healthy periodontal status（control group） who underwent prenatal examinations at Cangzhou People's Hospital and had a gestational age <26 weeks were enrolled. The clinical data and high-sensitivity C-reactive protein（hs-CRP）determination results were collected，and serum HMGB1 and IL-6 levels were determined. The subjects were classified into favorable and adverse outcome groups. Multivariate Logistic regression analysis was used to evaluate the influencing factors of adverse pregnancy outcomes in pregnant women with periodontal disease. Receiver operating characteristic（ROC） curve was used to evaluate the efficacy of each indicator in judging the adverse pregnancy outcomes of pregnant women with periodontal disease. Results Serum HMGB1 and IL-6 levels in periodontal disease group were higher than those in control group（P<0.001）. The proportions of preterm infants，low birth weight infants and small for gestational age infants in periodontal disease group were all higher than those in control group（P<0.05），and the new born weight was lower than that in control group（P<0.001）. The proportions of complications（gestational diabetes，gestational hypertension，gestational anemia，preeclampsia） and the levels of hs-CRP，HMGB1 and IL-6 in adverse outcome group were higher than those in favorable outcome group（P<0.05）. Gestational diabetes，gestational hypertension，gestational anemia，preeclampsia and elevated hs-CRP，HMGB1 and IL-6 were all risk factors for adverse pregnancy outcomes in pregnant women with periodontal disease（P<0.05）. Based on the results of multivariate Logistic regression analysis，a combined prediction model for judging the adverse pregnancy outcomes of pregnant women with periodontal disease was constructed. The areas under curves（AUC） of serum HMGB1，IL-6 single determinations and the combined prediction model for judging the adverse pregnancy outcomes of pregnant women with periodontal disease were 0.768，0.766 and 0.839，respectively. Conclusions Elevated levels of HMGB1 and IL-6 can increase the risk of adverse pregnancy outcomes in pregnant women with periodontal disease. Serum HMGB1 and IL-6 may serve as biomarkers for evaluating the pregnancy outcomes of pregnant women with periodontal disease.

Key words: High mobility group protein B1, Interleukin-6, Periodontal disease, Pregnant woman, Pregnancy outcome

CLC Number:

R446.1

WEI Yuexiao, YANG Lili, GUO Fengtong. Influence of serum HMGB1 and IL-6 levels on pregnancy outcomes of pregnant women with periodontal disease[J]. Laboratory Medicine, 2025, 40(10): 982-986.

Figures/Tables 6

References 19

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组别	例数	HMGB1/（ng·L^-1）	IL-6/（ng·L^-1）
牙周病组	200	794.42±170.18	48.85±9.72
对照组	100	616.75±157.72	39.04±7.05
t值		8.731	8.978
P值		<0.001	<0.001

组别	例数	HMGB1/（ng·L^-1）	IL-6/（ng·L^-1）
牙周病组	200	794.42±170.18	48.85±9.72
对照组	100	616.75±157.72	39.04±7.05
t值		8.731	8.978
P值		<0.001	<0.001

组别	例数	新生儿体重/kg	早产儿/[例（%）]	低体重儿/[例（%）]	胎儿窒息/[例（%）]	小于胎龄儿/[例（%）]
牙周病组	200	3.18±0.24	52（26.00）	57（28.50）	7（3.50）	55（27.50）
对照组	100	3.31±0.16	15（15.00）	12（12.00）	4（4.00）	13（13.00）
统计值		4.898	4.651	10.248	0.047	7.996
P值		<0.001	0.031	0.001	0.828	0.005

组别	例数	新生儿体重/kg	早产儿/[例（%）]	低体重儿/[例（%）]	胎儿窒息/[例（%）]	小于胎龄儿/[例（%）]
牙周病组	200	3.18±0.24	52（26.00）	57（28.50）	7（3.50）	55（27.50）
对照组	100	3.31±0.16	15（15.00）	12（12.00）	4（4.00）	13（13.00）
统计值		4.898	4.651	10.248	0.047	7.996
P值		<0.001	0.031	0.001	0.828	0.005

组别	例数	年龄/岁	BMI/ （kg·m^-2）	孕次/次	产次/次	分娩孕周/ 周	分娩方式
组别	例数	年龄/岁	BMI/ （kg·m^-2）	孕次/次	产次/次	分娩孕周/ 周	顺产/[例（%）]		剖宫产/[例（%）]
结局良好组	126	28.71±3.73	26.94±2.05	2±1	1±0	38.78±1.75	79（62.70）		47（37.30）
结局不良组	74	29.04±4.05	26.47±2.13	2±1	1±0	39.05±1.62	45（60.81）		29（39.19）
统计值		0.585	1.543	0.000	0.000	1.082	0.071
P值		0.559	0.124	1.000	1.000	0.28	0.791
组别	并发症						hs-CRP/ （mg·L^-1）	HMGB1/ （ng·L^-1）		IL-6/ （ng·L^-1）
组别	妊娠糖尿病/[例（%）]		妊娠高血压/[例（%）]		妊娠期贫血/[例（%）]	子痫前期/ [例（%）]	hs-CRP/ （mg·L^-1）	HMGB1/ （ng·L^-1）		IL-6/ （ng·L^-1）
结局良好组	24（19.05）		12（9.52）		13（10.32）	5（3.97）	2.34±0.41	737.05±148.34		45.74±7.39
结局不良组	25（33.78）		15（20.27）		16（21.62）	9（12.16）	2.59±0.37	892.10±157.89		54.15±8.31
统计值	5.473		4.611		4.805	4.808	4.314	6.968		7.417
P值	0.019		0.032		0.028	0.028	<0.001	<0.001		<0.001