Abstract:

Objective To analyze the correlation of seminal plasma silent information regulator 1（SIRT1） protein expression level with sperm parameters and ages. Methods A total of 110 male patients of 21-40-year-old from the andrology departments of Tongren Hospital of Shanghai Jiao Tong University School of Medicine and Shanghai Seventh People's Hospital were enrolled,and their semen analysis and seminal plasma SIRT1 protein expression level were performed and determined. The relation was evaluated among age,sperm parameters and seminal plasma SIRT1 protein expression levels. The control group was composed of 60 patients with normal sperm parameters,and the abnormal group was consisted of 50 patients with abnormal sperm parameters. The research subjects were classified into 21-30-year-old and 31-40-year-old groups. Seminal plasma SIRT1 protein expression levels in these groups were compared. Results The expression level of seminal plasma SIRT1 protein was positively correlated with age,sperm concentration and progressive motility sperm rate（PR）（r values were 0.28,0.42 and 0.29,P<0.01）. According to different ages,seminal plasma SIRT1 protein expression level in abnormal group was lower than that in control group（P<0.01）. In 31-40-year-old group,seminal plasma SIRT1 protein expression level in abnormal group was lower than that in control group（P<0.05）. According to different sperm parameters,seminal plasma SIRT1 protein expression level in 31-40-year-old group was higher than that in 21-30-year-old group（P<0.01）,and it of 31-40-year-old patients was also higher than that of 21-30-year-old patients in control group（P<0.01）. Conclusions Seminal plasma SIRT1 protein expression is related to ages and sperm parameters,decreasing with sperm quantity and quality and increasing with age,which could be utilized to assess male reproductive potential.

Key words: Silent information regulator 1, Sperm parameter, Age