Laboratory Medicine ›› 2022, Vol. 37 ›› Issue (3): 288-290.DOI: 10.3969/j.issn.1673-8640.2022.03.019
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Received:2020-09-17
Revised:2021-03-26
Online:2022-03-30
Published:2022-05-10
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| 样品编号 | 稀释方案 | 结果1/（pg/mL） | 结果2/（pg/mL） | 结果3/（pg/mL） | 均值/（pg/mL） | CV/% |
|---|---|---|---|---|---|---|
| 1 | L | 113.98 | 112.64 | 115.40 | 114.01 | 1.21 |
| 2 | 0.8L+0.2H | 1 141.75 | 1 145.19 | 1 116.74 | 1 134.56 | 1.37 |
| 3 | 0.6L+0.4H | 2 053.85 | 2 011.27 | 2 078.93 | 2 048.02 | 1.67 |
| 4 | 0.4L+0.6H | 2 825.17 | 2 874.35 | 2 812.38 | 2 837.30 | 1.15 |
| 5 | 0.2L+0.8H | 3 614.56 | 3 553.70 | 3 580.81 | 3 583.02 | 0.85 |
| 6 | H | 4 308.44 | 4 454.35 | 4 317.68 | 4 360.16 | 1.87 |
| 样品编号 | 稀释方案 | 结果1/（pg/mL） | 结果2/（pg/mL） | 结果3/（pg/mL） | 均值/（pg/mL） | CV/% |
|---|---|---|---|---|---|---|
| 1 | L | 113.98 | 112.64 | 115.40 | 114.01 | 1.21 |
| 2 | 0.8L+0.2H | 1 141.75 | 1 145.19 | 1 116.74 | 1 134.56 | 1.37 |
| 3 | 0.6L+0.4H | 2 053.85 | 2 011.27 | 2 078.93 | 2 048.02 | 1.67 |
| 4 | 0.4L+0.6H | 2 825.17 | 2 874.35 | 2 812.38 | 2 837.30 | 1.15 |
| 5 | 0.2L+0.8H | 3 614.56 | 3 553.70 | 3 580.81 | 3 583.02 | 0.85 |
| 6 | H | 4 308.44 | 4 454.35 | 4 317.68 | 4 360.16 | 1.87 |
| 阶数 | 方程因子 | 方程因子值 | 斜率标准误 | t值 | 回归标准误差 | 是否为最适方程 |
|---|---|---|---|---|---|---|
| 一阶 | b0 | 248.65 | 78.98 | 3.15 | 109.12 | 否 |
| b1 | 41.95 | 1.30 | 32.16 | |||
| 二阶 | b0 | 136.26 | 37.72 | 3.61 | 41.61 | 否 |
| b1 | 50.38 | 1.77 | 28.40 | |||
| b2 | -0.08 | 0.02 | -4.95 | |||
| 三阶 | b0 | 110.31 | 12.87 | 8.57 | 13.13 | 是 |
| b1 | 56.31 | 1.25 | 45.05 | |||
| b2 | -0.25 | 0.03 | -7.94 | |||
| b3 | 0.00 | 0.00 | 5.30 |
| 阶数 | 方程因子 | 方程因子值 | 斜率标准误 | t值 | 回归标准误差 | 是否为最适方程 |
|---|---|---|---|---|---|---|
| 一阶 | b0 | 248.65 | 78.98 | 3.15 | 109.12 | 否 |
| b1 | 41.95 | 1.30 | 32.16 | |||
| 二阶 | b0 | 136.26 | 37.72 | 3.61 | 41.61 | 否 |
| b1 | 50.38 | 1.77 | 28.40 | |||
| b2 | -0.08 | 0.02 | -4.95 | |||
| 三阶 | b0 | 110.31 | 12.87 | 8.57 | 13.13 | 是 |
| b1 | 56.31 | 1.25 | 45.05 | |||
| b2 | -0.25 | 0.03 | -7.94 | |||
| b3 | 0.00 | 0.00 | 5.30 |
| 样品稀释方案 | 一阶拟合值 | 二阶拟合值 | 三阶拟合值 | [P（x）-（b0+b1x）]2 | ADL（临界值为5.4%） |
|---|---|---|---|---|---|
| L | 248.65 | 136.26 | 110.31 | 19 137.33 | 3.78%<5.4%临床可接受的非线性 |
| 0.8L+0.2H | 1 087.66 | 1 110.14 | 1 146.47 | 3 459.01 | |
| 0.6L+0.4H | 1 926.67 | 2 016.58 | 2 037.34 | 12 247.89 | |
| 0.4L+0.6H | 2 765.68 | 2 855.59 | 2 834.83 | 4 780.68 | |
| 0.2L+0.8H | 3 604.70 | 3 627.17 | 3 590.84 | 192.10 | |
| H | 4 443.71 | 4 331.32 | 4 357.28 | 7 469.82 |
| 样品稀释方案 | 一阶拟合值 | 二阶拟合值 | 三阶拟合值 | [P（x）-（b0+b1x）]2 | ADL（临界值为5.4%） |
|---|---|---|---|---|---|
| L | 248.65 | 136.26 | 110.31 | 19 137.33 | 3.78%<5.4%临床可接受的非线性 |
| 0.8L+0.2H | 1 087.66 | 1 110.14 | 1 146.47 | 3 459.01 | |
| 0.6L+0.4H | 1 926.67 | 2 016.58 | 2 037.34 | 12 247.89 | |
| 0.4L+0.6H | 2 765.68 | 2 855.59 | 2 834.83 | 4 780.68 | |
| 0.2L+0.8H | 3 604.70 | 3 627.17 | 3 590.84 | 192.10 | |
| H | 4 443.71 | 4 331.32 | 4 357.28 | 7 469.82 |
| 样品稀释方案 | 测量均值/（pg/mL） | 一阶拟合值 | 二阶拟合值 | 三阶拟合值 | 每个浓度处线性偏离/% | 设定的误差范围/% （TEa=25%） | 线性判断 |
|---|---|---|---|---|---|---|---|
| L | 114.01 | 248.65 | 136.26 | 110.31 | 121.34 | 8 | 非线性 |
| 0.8L+0.2H | 1 134.56 | 257.04 | 146.34 | 121.56 | 11.94 | 8 | 非线性 |
| 0.6L+0.4H | 2 048.02 | 265.43 | 156.40 | 132.79 | 6.48 | 8 | 线性 |
| 0.4L+0.6H | 2 837.30 | 273.82 | 166.46 | 144.00 | -4.58 | 8 | 线性 |
| 0.2L+0.8H | 3 583.02 | 282.21 | 176.51 | 155.20 | 3.54 | 8 | 线性 |
| H | 4 360.16 | 290.60 | 186.56 | 166.37 | 2.85 | 8 | 线性 |
| 样品稀释方案 | 测量均值/（pg/mL） | 一阶拟合值 | 二阶拟合值 | 三阶拟合值 | 每个浓度处线性偏离/% | 设定的误差范围/% （TEa=25%） | 线性判断 |
|---|---|---|---|---|---|---|---|
| L | 114.01 | 248.65 | 136.26 | 110.31 | 121.34 | 8 | 非线性 |
| 0.8L+0.2H | 1 134.56 | 257.04 | 146.34 | 121.56 | 11.94 | 8 | 非线性 |
| 0.6L+0.4H | 2 048.02 | 265.43 | 156.40 | 132.79 | 6.48 | 8 | 线性 |
| 0.4L+0.6H | 2 837.30 | 273.82 | 166.46 | 144.00 | -4.58 | 8 | 线性 |
| 0.2L+0.8H | 3 583.02 | 282.21 | 176.51 | 155.20 | 3.54 | 8 | 线性 |
| H | 4 360.16 | 290.60 | 186.56 | 166.37 | 2.85 | 8 | 线性 |
| 相对量 | 0.00 | 1.00 | 2.00 | 3.00 | 4.00 | 5.00 |
|---|---|---|---|---|---|---|
| 检测均值 | 114.01 | 1 134.56 | 2 048.02 | 2 837.30 | 3 583.02 | 4 360.16 |
| 减114.01① | 0 | 1 020.55 | 1 934.01 | 2 723.29 | 3 469.02 | 4 246.15 |
| 斜率 | 1 020.55 | 967.00 | 907.76 | 867.25 | 849.23 | |
| 平均斜率（中位数②） | 907.76 | |||||
| 相对变化（x） | 907.76 | 1 815.53 | 2 723.29 | 3 631.06 | 4 538.82 | |
| N③ | 6 | 9 | 12 | 15 | ||
| 截距b0 | 221.10 | 303.89 | 367.06 | 396.75 | ||
| 斜率b1 | 1.006 3 | 0.937 9 | 0.896 1 | 0.879 8 | ||
| r2 | 0.997 8 | 0.997 1 | 0.997 1 | 0.997 5 | ||
| Y的均值 | 1 134.56 | 2 048.02 | 2 837.3 | 3 583.02 | 4 360.16 |
| 相对量 | 0.00 | 1.00 | 2.00 | 3.00 | 4.00 | 5.00 |
|---|---|---|---|---|---|---|
| 检测均值 | 114.01 | 1 134.56 | 2 048.02 | 2 837.30 | 3 583.02 | 4 360.16 |
| 减114.01① | 0 | 1 020.55 | 1 934.01 | 2 723.29 | 3 469.02 | 4 246.15 |
| 斜率 | 1 020.55 | 967.00 | 907.76 | 867.25 | 849.23 | |
| 平均斜率（中位数②） | 907.76 | |||||
| 相对变化（x） | 907.76 | 1 815.53 | 2 723.29 | 3 631.06 | 4 538.82 | |
| N③ | 6 | 9 | 12 | 15 | ||
| 截距b0 | 221.10 | 303.89 | 367.06 | 396.75 | ||
| 斜率b1 | 1.006 3 | 0.937 9 | 0.896 1 | 0.879 8 | ||
| r2 | 0.997 8 | 0.997 1 | 0.997 1 | 0.997 5 | ||
| Y的均值 | 1 134.56 | 2 048.02 | 2 837.3 | 3 583.02 | 4 360.16 |
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