Abstract:

Objective To investigate the application role of next-generation sequencing（NGS） in the diagnosis of difficult pathogen infection. Methods Totally,2 samples of ascites and 4 samples of peritoneal dialysis fluid were collected from a patient with stage 5 chronic kidney disease complicated with abdominal pain. The morphology of sediments was observed by microscopy directly. Peritoneal dialysis fluid and ascites were cultured using routine method. Toxoplasma IgG and IgM antibodies were detected by chemiluminescence immunoassay. NGS was used to detect the pathogenic genes from peritoneal dialysis fluid and ascites samples. Results The suspected Toxoplasma pseudocapsule was found by microscopy. Microbial culture was showed aseptic growth. Toxoplasma IgG and IgM antibodies were negative reaction. NGS showed there was Toxoplasma gondii infection in the peritoneal dialysis fluid. Conclusions NGS has advantages and application prospects in the diagnosis and treatment guidance of difficult pathogen infection.

Key words: Toxoplasma gondii, Peritoneal dialysis fluid, Infection, Next-generation sequencing