Research progress and application of RNase H-dependent PCR technology

doi:10.3969/j.issn.1673-8640.2021.012.017

Abstract

Abstract:

Ribonuclease（RNase） H-dependent polymerase chain reaction（PCR）（rhPCR） adds RNase H2 and blocked cleavable rhPCR primers based on PCR,which eliminates the formation of primer-dimers in the reaction process,greatly reduces the occurrence of error amplification,and significantly improves the specificity,sensitivity and repeatability of the reaction. The rhPCR shows certain advantages in single nucleotide polymorphism（SNP）,genotyping,multi-target simultaneous detection,environmental nucleic acid detection and so on. This review summarizes the background,principles,key technology,technical advantages,application and development prospects of rhPCR.

Key words: Ribonuclease H-dependent polymerase chain reaction, Blocked cleavable primer, Ribonuclease H2

CLC Number:

R446.1

WU Xinxin, HAN Mingyue, YU Daojun. Research progress and application of RNase H-dependent PCR technology[J]. Laboratory Medicine, 2021, 36(12): 1277-1282.

References 40

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