Abstract:

Objective To investigate the diagnostic role of combined detection of serum adenosine deaminase（ADA）and lactate dehydrogenase（LDH） and CD4⁺/CD8⁺ ratio and CD19⁺B lymphocyte percentage in peripheral blood in infectious mononucleosis（IM）. Methods A total of 78 children with IM were enrolled as experimental group,80 healthy children were enrolled as healthy control group,and 88 children with bronchopneumonia or acute upper respiratory tract infection were enrolled as disease control group. Blood specimens of children from experimental group and disease control group were collected at the first visit. Serum levels of ADA and LDH were determined by peroxidase method and rate method,and the CD4⁺/CD8⁺ ratio and the percentage of CD19⁺B lymphocyte in peripheral blood were determined by flow cytometry. Linear correlation analysis was used to analyze the correlation of serum ADA and LDH levels in IM group and the correlation of peripheral blood CD4⁺/CD8⁺ ratio and CD19⁺B lymphocyte percentage. Receiver operating characteristic（ROC） curve analysis was used,and diagnostic efficiency was evaluated. Results Serum ADA and LDH in experimental group were higher than those in control groups（P<0.01）,and there was a positive correlation between them（r=0.445,P<0.01）. The CD4⁺/CD8⁺ ratio and the percentage of CD19⁺B lymphocyte in experimental group were lower than those in control groups（P<0.01）,and there was a positive correlation between them（r=0.681,P<0.01）. The areas under curves of ADA,LDH,CD4⁺/CD8⁺ ratio and the percentage of CD19⁺B lymphocyte were 0.983,0.911,0.992 and 0.986,respectively. The area under curve of the combined detection was 0.998. Conclusions There are high sensitivity and specificity of the combined detection of serum ADA and LDH and the percentage of peripheral blood lymphocyte subsets in diagnosing IM,and it is beneficial for the early diagnosis of IM.

Key words: Adenosine deaminase, CD4⁺/CD8⁺ ratio, Infectious mononucleosis