Identification of fibrinolytic enzyme-producing strain and optimization of producing enzyme conditions

doi:10.3969/j.issn.1673-8640.2017.04.021

Abstract

Abstract:

Objective To identify fibrinolytic enzyme-producing strain （LZ-01） and optimize producing enzyme conditions. Methods The morphological characteristics of LZ-01 were observed. Physiological and biochemical tests and the sequence similarity analysis of phoR gene were adopted to determine the strain. Through single factor experiment,the influence of carbon source and nitrogen source on enzyme activity was investigated,and the optimal conditions of enzyme production were determined by orthogonal experiment. Results After the identification,the similarity of this strain and Bacillus amyloliquefaciens standard strain FZ42 was 99.78%. So,the strain was identified as Bacillus amyloliquefaciens. After optimizing,the producing enzyme activity increased by 3.63 times. Conclusions This study provides a theoretical basis for the further development of fibrinolytic drugs.

Key words: Fibrinolytic enzyme, Identification, Bacillus amyloliquefaciens, Optimization, Single factor experiment, Orthogonal experiment

CLC Number:

R446.5

LI Lizhuo, HUANG Huanjing, LI Zhuowei, ZHENG Mei. Identification of fibrinolytic enzyme-producing strain and optimization of producing enzyme conditions[J]. Laboratory Medicine, 2017, 32(4): 338-342.

Figures/Tables 4

References 14

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项目	LZ-01菌株	项目	LZ-01菌株
硫化氢试验	-	淀粉水解试验	+
脓青素产生试验	-	纤维素水解试验	-
丙二酸利用试验	-	亚硝酸盐还原试验	+
柠檬酸盐试验	+	3-酮基乳糖试验	+
糖醇发酵试验	+	反硝化试验	-
甲基红试验	-	脲酶试验	-
V.P.试验	-	产氨试验	-
接触酶	+	吲哚试验	-

项目	LZ-01菌株	项目	LZ-01菌株
硫化氢试验	-	淀粉水解试验	+
脓青素产生试验	-	纤维素水解试验	-
丙二酸利用试验	-	亚硝酸盐还原试验	+
柠檬酸盐试验	+	3-酮基乳糖试验	+
糖醇发酵试验	+	反硝化试验	-
甲基红试验	-	脲酶试验	-
V.P.试验	-	产氨试验	-
接触酶	+	吲哚试验	-

序列号	种名	菌种号	相似性（%）
JN580984	萎缩芽孢杆菌	NRS-213T	70.7
JN580983	萎缩芽孢杆菌	SB512	38.4
JN580982	解淀粉芽孢杆菌	CU8004	92.3
JN580979	解淀粉芽孢杆菌	FZ42	97.8
CP002183	枯草芽孢杆菌	str.W23	40.6
JN580970	枯草芽孢杆菌	MAS88	69.4
JN580925	枯草芽孢杆菌	BD170	69.5
JN580971	枯草芽孢杆菌	ATCC 21332	69.6
JN580924	枯草芽孢杆菌	NDH03	69.4
JN580981	枯草芽孢杆菌	168	69.4
JN580976	地衣芽孢杆菌	ATCC 8480	36.9
JN580977	地衣芽孢杆菌	ATCC 11946	36.8
JN580978	短小芽孢杆菌	ATCC 7061	34.1
CP000813	短小芽孢杆菌	SAFR032	33.7
JN580980	短小芽孢杆菌	Biosubtyl	81.2
JN575333.1	苏云金芽孢杆菌	HD2	33.3
NC_006274.1	蜡状芽孢杆菌	E33L	33.3
JN580985.1	坚强芽孢杆菌	NRS854	32.9
JN580986.1	坚强芽孢杆菌	NRS613	33.2

序列号	种名	菌种号	相似性（%）
JN580984	萎缩芽孢杆菌	NRS-213T	70.7
JN580983	萎缩芽孢杆菌	SB512	38.4
JN580982	解淀粉芽孢杆菌	CU8004	92.3
JN580979	解淀粉芽孢杆菌	FZ42	97.8
CP002183	枯草芽孢杆菌	str.W23	40.6
JN580970	枯草芽孢杆菌	MAS88	69.4
JN580925	枯草芽孢杆菌	BD170	69.5
JN580971	枯草芽孢杆菌	ATCC 21332	69.6
JN580924	枯草芽孢杆菌	NDH03	69.4
JN580981	枯草芽孢杆菌	168	69.4
JN580976	地衣芽孢杆菌	ATCC 8480	36.9
JN580977	地衣芽孢杆菌	ATCC 11946	36.8
JN580978	短小芽孢杆菌	ATCC 7061	34.1
CP000813	短小芽孢杆菌	SAFR032	33.7
JN580980	短小芽孢杆菌	Biosubtyl	81.2
JN575333.1	苏云金芽孢杆菌	HD2	33.3
NC_006274.1	蜡状芽孢杆菌	E33L	33.3
JN580985.1	坚强芽孢杆菌	NRS854	32.9
JN580986.1	坚强芽孢杆菌	NRS613	33.2

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