Abstract:

Objective To analyze the influence of the percentage of spermatozoa with normal morphology on in vitro fertilization（IVF）embryo outcome after optimized semen treatment,and to provide a reference for clinical treatment. Methods A total of 255 cases of IVF cycles were analyzed in which infertile was caused by women's fallopian tube disease,and oocytes were obtained ≥3. The semen optimized for IVF treatment was used. According to the related standards of World Health Organization,optimized semen were stained by modified Pap staining method and morphological analysis. According to the percentage of spermatozoa with normal morphology,they were classified into 2 groups,group A ≤19.03% and group B >19.03%. The fertilization rate,cleavage rate,available embryo rate,abnormal fertilization rate and high-quality embryo rate between group A and group B were compared. Results The fertilization rates of group A and group B were 77.8% and 79.6%,the cleavage rates were 95.4% and 96.0%,the available embryo rates were 82.0% and 85.4%,and the abnormal fertilization rates were 8.3% and 7.7%,respectively. The high-quality embryo rates were 71.4% and 71.6%,respectively. There was no statistical significance between the 2 groups in fertilization rate,cleavage rate,abnormal fertilization rate and high-quality embryo rate（P=0.247,0.461,0.549 and 0.958,respectively）. The available embryo rate of group B was higher than that of group A（P=0.038）. Conclusions The percentage of optimized spermatozoa with normal morphology can affect embryo outcome in IVF cycles. Early intervention is necessary to obtain good embryo outcome.

Key words: Semen optimization, Sperm morphology, In vitro fertilization, Embryo outcome