Correlations of cognitive impairment with vitamin B12 and folic acid in elderly dementia patients

doi:10.3969/j.issn.1673-8640.2017.04.009

Abstract

Abstract:

Objective To investigate the correlations of vitamin B₁₂ and folic acid with mild cognitive impairment （MCI） and Alzheimer's disease（AD）. Methods Serum vitamin B₁₂ and folic acid levels were determined by chemiluminescence microparticle immunoassay in 191 elderly dementia patients ≥ 50 years old and 69 cognitive normal subjects（normal control group）. The low levels of vitamin B₁₂ and folic acid as high-risk factors for AD were analyzed. Cognitive function was evaluated with comprehensive neuropsychological tests,such as Chinese Version of Mini Mental State Examination（CMMSE） and the Memory and Executive Screening（MES）. Pearson's correlation analysis was performed to evaluate the relationship among serum vitamin B₁₂,folic acid,age,education year,body mass index（BMI） and cognitive function in all groups. Logistic regression was used to evaluate the association of vitamin B₁₂ and folic acid with MCI and AD. Results A total of 54 patients with MCI and 137 patients with AD were determined. There were 78.1% patients with normal vitamin B₁₂ and folic acid levels,6.1% patients with low vitamin B₁₂ level,5.8% patients with low folic acid level,and 10.0% with high vitamin B₁₂ or folic acid level. Vitamin B₁₂ or folic acid level had no correlation with CMMSE and MES scores after covariates' adjustments（P>0.05）. Low vitamin B₁₂ level was correlated with the risk of AD [odds ratio（OR）=2.869,95% confidence interval（CI）1.123-7.333]. Conclusions Vitamin B₁₂ and folic acid are not correlated with cognitive impairment. However,low vitamin B₁₂ level may be correlate with an increased risk for AD.

Key words: Vitamin B12, Folic acid, Alzheimer's disease, Cognitive impairment

CLC Number:

R446.1

HU Yao, GUO Qihao, CEN Yi, LIN Yao. Correlations of cognitive impairment with vitamin B₁₂ and folic acid in elderly dementia patients[J]. Laboratory Medicine, 2017, 32(4): 284-289.

Figures/Tables 6

References 20

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组别	例数		年龄（岁）		性别（例）			BMI（kg/m²）		CMMSE评分（分）
组别	例数		年龄（岁）		男		女	BMI（kg/m²）		CMMSE评分（分）
MCI组	54		64.39±9.63		32		22	23.47±3.12		64.28±11.18^*#
AD组	137		63.36±9.49		81		56	23.51±3.20		32.68±19.15^*
正常对照组	69		63.91±10.30		34		35	23.68±3.15		84.17±6.33
P值			0.790		0.366			0.079		<0.001
组别		MES评分（分）		受教育年限（年）		维生素B₁₂（pg/mL）			叶酸（ng/mL）
MCI组		26.17±1.79^*#		9.45±3.43		580.12±370.91			8.08±4.36
AD组		14.94±5.97^*		8.78±4.16		499.06±219.47^*			7.92±4.19
正常对照组		27.96±1.40		9.90±3.24		638.32±352.60			9.05±3.83
P值		<0.001		0.121		0.005			0.170

组别	例数		年龄（岁）		性别（例）			BMI（kg/m²）		CMMSE评分（分）
组别	例数		年龄（岁）		男		女	BMI（kg/m²）		CMMSE评分（分）
MCI组	54		64.39±9.63		32		22	23.47±3.12		64.28±11.18^*#
AD组	137		63.36±9.49		81		56	23.51±3.20		32.68±19.15^*
正常对照组	69		63.91±10.30		34		35	23.68±3.15		84.17±6.33
P值			0.790		0.366			0.079		<0.001
组别		MES评分（分）		受教育年限（年）		维生素B₁₂（pg/mL）			叶酸（ng/mL）
MCI组		26.17±1.79^*#		9.45±3.43		580.12±370.91			8.08±4.36
AD组		14.94±5.97^*		8.78±4.16		499.06±219.47^*			7.92±4.19
正常对照组		27.96±1.40		9.90±3.24		638.32±352.60			9.05±3.83
P值		<0.001		0.121		0.005			0.170

组别	例数		年龄（岁）		性别（例）			BMI（kg/m²）
组别	例数		年龄（岁）		男	女		BMI（kg/m²）
低叶酸	15		61.73±8.77		6	9		22.08±3.21
低维生素B₁₂	16		65.44±8.29		9	7		23.07±3.01
正常维生素B₁₂和叶酸	203		63.91±10.00		110	93		23.11±3.11
高维生素B₁₂或叶酸	26		62.31±8.77		12	14		23.14±3.15
P值			0.627		0.341			0.745
组别		CMMSE评分（分）		MES评分（分）			受教育年限（年）
低叶酸		20.20±5.92		49.13±24.71			9.43±4.19
低维生素B₁₂		19.62±8.29		49.50±27.62			8.56±3.76
正常维生素B₁₂和叶酸		20.41±7.75		51.44±27.40			9.29±3.78
高维生素B3或叶酸		24.15±6.12		52.91±27.07			8.96±4.01
P值		0.109		0.130			0.874

组别	例数		年龄（岁）		性别（例）			BMI（kg/m²）
组别	例数		年龄（岁）		男	女		BMI（kg/m²）
低叶酸	15		61.73±8.77		6	9		22.08±3.21
低维生素B₁₂	16		65.44±8.29		9	7		23.07±3.01
正常维生素B₁₂和叶酸	203		63.91±10.00		110	93		23.11±3.11
高维生素B₁₂或叶酸	26		62.31±8.77		12	14		23.14±3.15
P值			0.627		0.341			0.745
组别		CMMSE评分（分）		MES评分（分）			受教育年限（年）
低叶酸		20.20±5.92		49.13±24.71			9.43±4.19
低维生素B₁₂		19.62±8.29		49.50±27.62			8.56±3.76
正常维生素B₁₂和叶酸		20.41±7.75		51.44±27.40			9.29±3.78
高维生素B3或叶酸		24.15±6.12		52.91±27.07			8.96±4.01
P值		0.109		0.130			0.874

组别	年龄（岁）	受教育年限（年）	BMI（kg/m²）	维生素B₁₂（pg/mL）	叶酸（ng/mL）
正常对照组
r值	-0.342	0.257	0.145	0.438	0.289
P值	0.129	0.187	0.110	0.167	0.198
MCI组
r值	-0.543	0.673	0.321	0.453	0.279
P值	0.198	0.101	0.297	0.187	0.168
AD组
r值	-0.368	0.198	0.278	0.215	0.193
P值	0.109	0.020	0.195	0.284	0.118

Correlations of cognitive impairment with vitamin B₁₂ and folic acid in elderly dementia patients

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组别	年龄（岁）	受教育年限（年）	BMI（kg/m²）	维生素B₁₂（pg/mL）	叶酸（ng/mL）
正常对照组
r值	-0.250	0.401	0.309	0.541	0.327
P值	0.038	0.001	0.158	0.108	0.209
MCI组
r值	-0.398	0.509	0.231	0.309	0.341
P值	0.102	0.198	0.209	0.198	0.897
AD组
r值	-0.381	0.119	0.501	0.187	0.203
P值	0.409	0.209	0.118	0.029^*	0.098

变量^*	组别	平均水平	P值	OR值	95%CI
维生素B₁₂（pg/mL）	Ⅰ	242.27	0.028	2.869	1.123~7.333
	Ⅱ	374.54	0.047	2.581	1.012~6.583
	Ⅲ	479.44	0.049	2.526	1.002~6.364
	Ⅳ	647.68	0.130	2.020	0.653~3.647
叶酸（ng/mL）	Ⅰ	3.56	0.071	1.298	0.248~6.656
	Ⅱ	5.52	0.085	1.273	0.254~6.826
	Ⅲ	7.69	0.905	1.263	0.228~6.994
	Ⅳ	9.83	0.652	1.235	0.150~9.367

变量^*	组别	平均水平	P值	OR值	95%CI
维生素B₁₂（pg/mL）	Ⅰ	312.53	0.135	1.536	0.492~4.893
	Ⅱ	450.38	0.159	1.297	0.249~6.780
	Ⅲ	585.75	0.263	1.083	0.197~6.135
	Ⅳ	731.50	0.265	1.063	0.172~9.378
叶酸（ng/mL）	Ⅰ	4.83	0.541	1.015	0.183~5.643
	Ⅱ	6.87	0.597	0.729	0.125~4.283
	Ⅲ	8.50	0.632	0.643	0.115~3.625
	Ⅳ	10.57	0.601	0.578	0.104~3.057

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