Expressions of natural killer-associated receptors on cytotoxic T lymphocyte from peripheral blood of patients with chronic hepatitis B

doi:10.3969/j.issn.1673-8640.2017.02.009

Abstract

Abstract:

Objective To investigate the change patterns of natural killer（NK）-associated receptors（NKR） on cytotoxic T lymphocyte（CTL）from peripheral blood of patients with chronic hepatitis B（CHB）,and to study the receptor pathway of CTL from CHB patients responding to hepatitis B virus（HBV）.Methods The percentages of CTL and the expressions of NK-associated activating receptors（NKG2D and NKp30） and NK-associated inhibitory receptors（NKG2A and KIR2DL3） on CTL were determined by flow cytometry in 40 patients with CHB（18 cases of HBV DNA positive and 22 cases of HBV DNA negative） and 20 healthy controls. The difference between the 2 groups and the correlations with HBV DNA load,HBV infection serology markers and liver injury markers and so on were analyzed.Results The percentage of CTL in T cells from peripheral blood in CHB group [（19.33±6.25）%] was lower than that of healthy control group[（23.94±6.09）%]（P<0.05）. The proportion of CTL expressing KIR2DL3 in CHB group [（3.32±2.63）%] was higher than that in healthy control group [（1.55±0.53）%]（P<0.05）. The percentage of CTL in HBV DNA positive CHB group [（16.94±4.8）%] was lower than that in HBV DNA negative CHB group[（21.29±6.7）%]（P<0.05）,and the proportion of CTL expressing KIR2DL3 in HBV DNA positive CHB group[（3.85±3.41）%] was higher than that in HBV DNA negative CHB group[（2.91±1.81）%]（P<0.05）. The proportion of CTL expressing NKG2A was positively correlated with HBV DNA load and alanine aminotransferase（ALT） level in CHB group （r=0.580 and 0.671,P<0.01）,and the proportion of CTL expressing KIR2DL3 was positively correlated with hepatitis B e antigen（HBeAg）（r=0.556,P<0.001）. Conclusions There is a defect in quantity and function of CTL in CHB patients,which means T cell exhaustion. The change pattern of NK-associated inhibitory receptors and activating receptors on CTL from CHB patients impairs the activation of CTL and immune response disorders,which results that HBV can not be effectively cleared,and hepatic cells are injured. The expression levels of NK-associated inhibitory receptors on CTL are expected to develop into a new indicator to evaluate the disease severity and the potential targets of therapy for CHB.

Key words: Natural killer-associated receptor, Chronic hepatitis B, Cytotoxic T lymphocyte

CLC Number:

R446.1

GE Danhong, XU Chong, YAO Yufeng. Expressions of natural killer-associated receptors on cytotoxic T lymphocyte from peripheral blood of patients with chronic hepatitis B[J]. Laboratory Medicine, 2017, 32(2): 108-113.

Figures/Tables 7

References 10

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