Abstract:

Objective To study the influence of lysyl oxidase（LOX） gene drop expression in human vaginal wall fibroblasts on the mRNA expressions of elastin （ELN） and matrix metalloproteinase-2（MMP-2）. Methods The primary-cultured human vaginal wall fibroblasts were classified into 3 groups,LOX gene drop expression group,negative control group and blank control group. LOX-shRNA lentiviruses were transfected into the fibroblasts of LOX gene drop expression group. Negative control lentiviruses were transfected into the fibroblasts of negative control group. The transfection efficiency was calculated. Flow cytometry sorted fibroblasts with green fluorescence,and some cells were collected and determined. Real-time fluorescence quantitation polymerase chain reaction（PCR） was used to determine the mRNA expressions of LOX,ELN andMMP-2. Results A total of 8 samples were expressed in the fibroblasts in LOX gene drop expression group,including 4 cases of premenopausal women and 4 cases of postmenopausal women. The fibroblasts in LOX gene drop expression group became smaller,the growth was slow,and the adherence decreased. The expression of LOX mRNA in LOX gene drop expression group was lower than those in negative and blank control groups （P<0.05）. The expression of ELN mRNA in LOX gene drop expression group was lower than those in negative and blank control groups,while the expression of MMP-2 mRNA was higher. There was no statistical significance for LOX mRNA,ELN mRNA and MMP-2 mRNA expressions between negative and blank control groups（P>0.05）. Conclusions The expression of ELN mRNA decreases,however,that of MMP-2 mRNA increased. LOXmay participate in the occurrence of pelvic organ prolapse（POP） by regulating the expressions of ELN mRNA and MMP-2 mRNA.

Key words: Lysyl oxidase, Human vaginal wall fibroblasts, Elastin, Matrix metalloproteinase-2, Pelvic organ prolapse