Progress of nucleic acid amplification techniques in Mycobacterium detection and drug resistance analysis

doi:10.3969/j.issn.1673-8640.2016.05.018

Abstract

Abstract:

With the rapid development of molecular biology and genome sequence decryption of Mycobacterium,especially Mycobacterium tuberculosis complex （MTBC）,it is possible to detect and identify Mycobacterium and analyze drug resistance by molecular biological methods. Nucleic acid amplification （NAA） techniques are the basis of molecular biology. Recently,many kinds of Mycobacterium identification methods based on NAA techniques are established and evaluated. This paper reviews the progress of these methods and assesses their clinical application prospects in Mycobacterium detection and drug resistance analysis.

Key words: Nucleic acid amplification techniques, Mycobacterium tuberculosis, Mycobacterium, Drug resistance

CLC Number:

R446.5

LIU Minyan, BAI Bing, CHEN Jin. Progress of nucleic acid amplification techniques in Mycobacterium detection and drug resistance analysis[J]. Laboratory Medicine, 2016, 31(5): 426-432.

References 47

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