Application significance of Xpert MTB/RIF assay on the detection of MTB

doi:10.3969/j.issn.1673-8640.2016.01.012

Abstract

Abstract:

Objective To investigate the clinical application significance of Xpert Mycobacterium tuberculosis（MTB）/rifampicin（RIF）assay in the diagnosis of tuberculosis. Methods A total of 302 patients with tuberculosis were determined by Xpert MTB/RIF assay,acid-fast stain and culture,respectively. The results were compared. Results All the samples were detected by Xpert MTB/RIF assay,acid-fast stain and culture,and the positive rates of sputum samples were 53.9%,33.0% and 37.2%. The positive rates of lavage fluid were 65.9%,22.7% and 29.5%. The positive rates of hydrothorax were 16.4%,1.5% and 3.0%. The positive rates of Xpert MTB/RIF assay for all types of samples were higher than those of acid-fast stain and culture （P<0.01）,and there was no statistical significance between acid-fast stain and culture （P >0.05）. The positive rate of Xpert MTB/RIF assay in lavage fluid was 65.9%（29/44）,that in sputum samples was 53.9%（103/191）,and that in hydrothorax was 16.4%（11/67）. The RIF resistance rates from Xpert MTB/RIF assay [22.1%（19/86）] had no statistical significance with those from standard drug susceptibility testing [20.9%（18/86）,P=0.00]. The coincidence rate was 96.5％（83/86）. Conclusions Compared with acid-fast stain and culture,Xpert MTB/RIF assay is rapid,simple and sensitive in the detection of MTB,and has a good application prospect.

Key words: Xpert Mycobacterium tuberculosis/rifampicin assay, Mycobacterium tuberculosis, Tuberculosis

CLC Number:

R446.5

LI Yan, ZHANG Tianhua, XIAN Xiaoping, WANG Xidi, CHEN Meiling, YANG Jian, WANG Rui, JIN Yan. Application significance of Xpert MTB/RIF assay on the detection of MTB[J]. Laboratory Medicine, 2016, 31(1): 52-55.

Figures/Tables 1

References 10

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10	(

Xpert MTB/RIF技术	比例法		合计
Xpert MTB/RIF技术	耐药	敏感	合计
耐药	17	2	19
敏感	1	66	67
合计	18	68	86

Xpert MTB/RIF技术	比例法		合计
Xpert MTB/RIF技术	耐药	敏感	合计
耐药	17	2	19
敏感	1	66	67
合计	18	68	86

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