The research progress in respiratory virus laboratory testing technology

doi:10.3969/j.issn.1673-8640.2015.08.021

Abstract

Abstract:

Respiratory virus is one of main factors that threatens human health, so it is significant to identify pathogenic microorganisms accurately and rapidly. This article introduces some traditional or new laboratory testing technologies that have been applied to respiratory virus determination nowdays, including virus isolation culture technique, immunological technique, nucleic acid sequence amplification technique, chip technology, next generation sequencing technique, mass spectrometry (MS) technique and so on. These technologies are applied into clinical diagnosis, curative effect observation, scientific research and investigation of respiratory virus by their characteristics.

Key words: Respiratory virus, Laboratory testing technology, Research progress

CLC Number:

R446.5

YE Xingchen, YANG Haiou, WANG Jing.. The research progress in respiratory virus laboratory testing technology[J]. Laboratory Medicine, 2015, 30(8): 861-865.

References 33

[1]	TIVELJUNG-LINDELL A, ROTZÉN-OSTLUND M, GUPTA S, et al. Development and implementation of a molecular diagnostic platform for daily rapid detection of 15 respiratory viruses[J]. J Med Virol, 2009, 81(1): 167-175.
[2]	金玉霞. 呼吸道病毒检测方法的进展及意义[J]. 中华医院感染学杂志, 2013, 23(4): 959-960.
[3]	MAHONY JB, PETRICH A, SMIEJA M.Molecular diagnosis of respiratory virus infections[J]. Crit Rev Clin Lab Sci, 2011, 48(5-6): 217-249.
[4]	高颖阳, 姜晨彦, 蒋露芳,等. 犬肾细胞系、人喉表皮癌细胞、非洲绿猴肾细胞混合培养检测常见呼吸道病毒和肠道病毒[J]. 中华传染病杂志, 2011, 29(6): 321-324.
[5]	汪千力, 居丽芳, 蒋露芳,等. MDCK、HEp-2、Vero细胞混合冻存适用于分离培养常见病毒研究[J]. 中华疾病控制杂志, 2012, 16(10): 827-832.
[6]	LASALA PR, BUFTON KK, ISMAIL N, et al.Prospective comparison of R-mix shell vial system with direct antigen tests and conventional cell culture for respiratory virus detection[J]. J Clin Virol, 2007, 38(3): 210-216.
[7]	王春, 赵百慧, 张泓,等. 上海市儿童下呼吸道感染常见病毒诊断方法比较[J]. 检验医学, 2011, 26(9): 589-592.
[8]	俞小卫, 王亚楠, 程宝金,等. 直接免疫荧光法病毒抗原测定在呼吸道感染性疾病诊断中的应用[J]. 检验医学,2013, 28(1): 76-79.
[9]	JARTTI T, SÖDERLUND-VENERMO M, HEDMAN K, et al. New molecular virus detection methods and their clinical value in lower respiratory tract infections in children[J]. Paediatr Respir Rev, 2013, 14(1): 38-45.
[10]	KRUNIC N, MERANTE F, YAGHOUBIAN S, et al.Advances in the diagnosis of respiratory tract infections: role of the Luminex xTAG respiratory viral panel[J]. Ann N Y Acad Sci, 2011, 1222: 6-13.
[11]	DABISCH-RUTHE M, VOLLMER T, ADAMS O, et al.Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay[J]. BMC Infect Dis, 2012, 12: 163.
[12]	PORITZ MA, BLASCHKE AJ, BYINGTON CL, et al.FilmArray, an automated nested multiplex PCR system for multi-pathogen detection: development and application to respiratory tract infection[J]. PLoS One, 2011, 6(10): e26047.
[13]	徐昌平, 卢亦愚, 冯燕,等. 基于DPO引物的多重RT-PCR技术检测常见呼吸道病毒[J]. 中国卫生检验杂志, 2013, 23(3): 658-660.
[14]	翁学军, 张甦, 杜英,等. 多重实时荧光定量PCR技术检测儿童呼吸道新发病毒方法的建立与应用[J]. 中华检验医学杂志, 2013, 36(6): 538-542.
[15]	李瑾, 毛乃颖, 秦萌,等. GeXP多重PCR技术同时检测12种常见呼吸道病毒[J]. 病毒学报, 2011, 27(6): 526-532.
[16]	BERNING L, ABERLE SW, SIMON B, et al.Evaluation of a multiplex ligation-dependent probe amplification assay for the detection of respiratory pathogens in oncological patients[J]. J Clin Virol, 2014, 60(2): 141-146.
[17]	CHUNG B, SHIN GW, PARK CK, et al.Rapid and sensitive detection of lower respiratory tract infections by stuffer-free multiplex ligation-dependent probe amplification[J]. Electrophoresis, 2014, 35(4): 511-514.
[18]	BAWAGE SS, TIWARI PM, PILLAI S, et al.Recent advances in diagnosis, prevention, and treatment of human respiratory syncytial virus[J]. Adv Virol, 2013, 2013: 595768.
[19]	ZHANG P, PENG P, WANG L, et al.G-scores: a method for identifying disease-causing pathogens with application to lower respiratory tract infections[J]. Stat Med, 2014, 33(16): 2814-2829.
[20]	隋竑弢, 王大燕, 舒跃龙. 呼吸道病毒多病原检测技术研究进展[J]. 病毒学报, 2013, 29(2): 238-244.
[21]	陈沁, 裘敏燕, 付永锋,等. 4种呼吸道病毒悬液芯片检测方法的建立[J]. 中国病原生物学杂志, 2013, 26(2): 97-101.
[22]	GRENINGER AL, CHEN EC, SITTLER T, et al.A metagenomic analysis of pandemic influenza A (2009 H1N1) infection in patients from North America[J]. PLoS One, 2010, 5(10): e13381.
[23]	TOWNSEND MB, DAWSON ED, MEHLMANN M, et al.Experimental evaluation of the FluChip diagnostic microarray for influenza virus surveillance[J]. J Clin Microbiol, 2006, 44(8): 2863-2871.
[24]	ZHU H, HU S, JONA G, et al.Severe acute respiratory syndrome diagnostics using a coronavirus protein microarray[J]. Proc Natl Acad Sci USA, 2006, 103(11): 4011-4016.
[25]	VOELKERDING KV, DAMES SA, DURTSCHI JD.Next-generation sequencing: from basic research to diagnostics[J]. Clin Chem, 2009, 55(4): 641-658.
[26]	BIALASIEWICZ S, MCVERNON J, NOLAN T, et al.Detection of a divergent parainfluenza 4 virus in an adult patient with influenza like illness using next-generation sequencing[J]. BMC Infect Dis, 2014, 14: 275.
[27]	SAMPATH R, RUSSELL KL, MASSIRE C, et al.Global surveillance of emerging influenza virus genotypes by mass spectrometry[J]. PLoS One, 2007, 2(5): e489.
[28]	NIEMEYER CM, ADLER M, WACKER R.Immuno-PCR: high sensitivity detection of proteins by nucleic acid amplification[J]. Trends Biotechnol, 2005, 23(4): 208-216.
[29]	PEREZ JW, VARGIS EA, RUSS PK, et al.Detection of respiratory syncytial virus using nanoparticle amplified immuno-polymerase chain reaction[J]. Anal Biochem, 2011, 410(1): 141-148.
[30]	JAYAGOPAL A, HALFPENNY KC, PEREZ JW, et al.Hairpin DNA-functionalized gold colloids for the imaging of mRNA in live cells[J]. J Am Chem Soc, 2010, 132(28): 9789-9796.
[31]	HUDSON SD, CHUMANOV G.Bioanalytical applications of SERS (surface-enhanced Raman spectroscopy)[J]. Anal Bioanal Chem, 2009, 394(3): 679-686.
[32]	DRISKELL JD, ZHU Y, KIRKWOOD CD, et al.Rapid and sensitive detection of rotavirus molecular signatures using surface enhanced Raman spectroscopy[J]. PLoS One, 2010, 5(4): e10222.
[33]	LIU Z, ZHANG P, TANG S, et al.Urine real-time polymerase chain reaction detection for children virus pneumonia with acute human cytomegalovirus infection[J]. BMC Infect Dis, 2014, 14: 245.