Research progress on the bacterial inoculation techniques in clinical microbiology laboratories

doi:10.3969/j.issn.1673-8640.2015.07.020

Abstract

Abstract:

In recent years, researches on the bacterial inoculation automation are booming. The commercialized automatic or semi-automatic microbial inoculation instruments are developing, such as, Robobact system, EasySpiral Pro and PREVI Isola. However, the experience of clinical application indicates that no instrument employed is satisfactory, let alone the accurate quantitative inoculation instrument, and they are hard to be popularized in clinical microbiology laboratories. Meanwhile, to develop a new method of accurate quantitative determination and to unify and standardize operating procedure, is the development tendency of quality control. Based on Bernoulli principle and fluid mechanics,a new-type instrument, called the multichannel microbial inoculation instument, is invented by Chinese scholars. It is hopeful to develop a new method for the quantitative determination for microbiology based on automatic technique. In this review, the research and development progress of bacterial inoculation technique in clinical microbiology laboratories are summarized.

Key words: Inoculation technique, Clinical microbiology, Bacterial culture, Automation, Quantitative analysis

CLC Number:

R446.5

WANG Jinghua, GE Ping, CHEN Rong, XU Rong, LIU Xuejie. Research progress on the bacterial inoculation techniques in clinical microbiology laboratories[J]. Laboratory Medicine, 2015, 30(7): 757-760.

References 19

[1]	KAMIYA A,KIKUCHI A,TOMITA Y,et al.PCR and PCR-RFLP techniques targeting the DNA topoisomerase Ⅱ gene for rapid clinical diagnosis of the etiologic agent of dermatophytosis[J].J Demlatol Sci,2004,34(1):35-48.
[2]	HEALY M,REECE K,WALTON D,et al.Identification to the species level and differentiation between strains of Aspergillus clinical isolates by automated repetitive-sequence-based PCR[J].J Clin Microbiol,2004,42(9):4016-4024.
[3]	王敬华,虞培娟,王科元.一种多通道自动化细菌快速接种仪及其使用方法:中国,zl2011102285280[P].2014-02-19.
[4]	陈知行,陈慧莉,康梅,等.Robobact System自动接种培养仪的临床应用评价[J].中国医疗设备,2008,23(7):58-60.
[5]	HENRY DI.Clinical microbiology procedures handbook[M]. 2nd edition. Washington:ASM Press, 2004:74-638.
[6]	HILL GB,SCHALKOWSKY S.Development and evaluation of the spiral gradient endpoint method for susceptibility testing of anaerobic gram-negative bacilli[J]. Rev Infect Dis,1990,12(Suppl 2):S200-S209.
[7]	PONG R,BOOST MV,O'DONOGHUE MM,et al. Spiral gradient endpoint susceptibility testing: a fresh look at a neglected technique[J]. J Antimicrob Chemother, 2010 ,65(9):1959-1963.
[8]	叶应妩,王毓三,申子瑜.全国临床检验操作规程 [M]. 3版.南京:东南大学出版社,2006:736-753.
[9]	WS 310-2009 医院供应室清洗消毒规范[S].北京:中国标准出版社,2009.
[10]	GLASSON JH,GUTHRIE LH,NIELSEN DJ,et al.Evaluation of an automated instrument for inoculating and spreading samples onto agar plates[J].J Clin Microbiol,2008,46(4):1281-1284.
[11]	GREUB G,PROD'HOM G. Automation in clinical bacteriology: what system to choose[J].Clin Microbiol Infect, 2011,17(5):655-660.
[12]	FROMENT P,MARCHANDIN H,VANDE PERRE H,et al.Automated versus manual sample inoculations in routine clinical microbiology: a performance evaluation of the fully automated InoqulA instrument[J]. J Clin Microbiol,2014,52(3):796-802.
[13]	NEBBAD-LECHANI B,EMIRIAN A,MAILLEBUAU F,et al.New procedure to reduce the time and cost of broncho-pulmonary specimen management using the Previ Isola® automated inoculation system[J]. J Microbiol Methods, 2013,95(3):384-388.
[14]	王春玉,陈中举,闫少珍,等.PREVI Isola自动接种仪的应用评价[J].国际检验医学杂志,2013,35(20):2748-2749.
[15]	FDA. Medical device recalls [EB/OL]. (2012-10-09)[2014-06-15]. .
[16]	魏志勇.微生物自动接种仪: 中国,发明专利号zl2011101084886[P].2011-11-09.
[17]	中华人民共和国卫生部.医院感染诊断标准(试行)[J]. 中华医学杂志,2001,87(5):61-67.
[18]	NORTON MD,SPILKIA AJ, GODOY VG.Antibiotic resistance acquired through a DNA damage-inducible response in Acinetobacter baumannii[J].J Bacteriol,2013,195(6): 1335-1345.
[19]	王敬华,虞培娟,王科元.封闭式微生物标本快速接种仪:中国,zl2012201064213[P].2013-12-04.