The variation and its clinical significance of Th1/Th2 and CD28+ cells from peripheral blood in patients with chronic glomerulonephritis

doi:10.3969/j.issn.1673-8640.2015.02.009

Abstract

Abstract: Objective

To investigate the possible mechanism of cellular immunology in the process of chronic glomerulonephritis (CGN) by the detection of Th1/Th2 ratio and CD28⁺ cell percentages from peripheral blood in patients with CGN.

Methods

Th1/Th2 ratio and CD28⁺ cell percentages were detected by flow cytometry from 50 patients with CGN and 30 healthy subjects (control group). SPSS 16.0 software was used to analyze the results.

Results

Compared with control group, the percentages of CD3⁺CD4⁺ cells and CD4/CD8 ratio of patients with CGN decreased significantly (P<0.05). Although there was no statistical significance in the percentages of Th1 and Th2 cells (P>0.05), yet Th1/Th2 ratio increased significantly (P<0.05). The percentages of CD28⁺ and CD4⁺CD28⁺ cells decreased significantly (P<0.01).

Conclusions

T cell immune function is disorder, and activation is restricted in patients with CGN. Their cellular immune function is low, and there exists imbalance between Th1 and Th2 cells. The detection of Th1/Th2 ratio and percentages of CD28⁺ cells from peripheral blood in patients with CGN is valuable to understand the immune state so as to provide the theoretical basis for immune therapy.

Key words: Th1/Th2 cell, CD28+ cell, Flow cytometry, Chronic glomerulonephritis

CLC Number:

R446.63

ZHOU Yunjiao, LI Peng, ZHANG Lihong, QIAN Yingjun, NIU Jianying. The variation and its clinical significance of Th1/Th2 and CD28⁺ cells from peripheral blood in patients with chronic glomerulonephritis[J]. Laboratory Medicine, 2015, 30(2): 141-144.

Figures/Tables 2

References 13

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组别	CD3⁺ (%)	CD3⁺CD4⁺ (%)	CD3⁺CD8⁺ (%)	Th1 (%)	Th2 (%)	CD4/CD8	Th1/Th2
对照组	65.69±6.38	37.43±5.07	23.50±7.24	9.90±3.12	1.45±0.59	1.85±1.00	7.62±2.91
CGN组	63.46±7.37	34.65±5.53^*	27.28±8.81	11.51±3.93	1.30±0.37	1.47±0.72^*	9.67±5.15^*

组别	CD3⁺ (%)	CD3⁺CD4⁺ (%)	CD3⁺CD8⁺ (%)	Th1 (%)	Th2 (%)	CD4/CD8	Th1/Th2
对照组	65.69±6.38	37.43±5.07	23.50±7.24	9.90±3.12	1.45±0.59	1.85±1.00	7.62±2.91
CGN组	63.46±7.37	34.65±5.53^*	27.28±8.81	11.51±3.93	1.30±0.37	1.47±0.72^*	9.67±5.15^*

组别	CD28⁺	CD4⁺CD28⁺	CD8⁺CD28⁺
对照组	56.69±6.82	34.04±4.87	8.35±3.47
CGN组	50.54±5.59^*	30.58±5.30^*	9.50±2.53

组别	CD28⁺	CD4⁺CD28⁺	CD8⁺CD28⁺
对照组	56.69±6.82	34.04±4.87	8.35±3.47
CGN组	50.54±5.59^*	30.58±5.30^*	9.50±2.53

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