Evaluation on the performance of Reflotron-Hemoglobin dry-chemistry system

doi:10.3969/j.issn.1673-8640.2014.05.020

Abstract

Abstract: Objective To evaluate Reflotron-Hemoglobin dry-chemistry system for the measurement of hemoglobin. Methods The within-run and between-run precisions, linearity, correlation and reference range of Reflotron- Hemoglobin dry-chemistry system was verified. Results The within-run precision was 2.54%-2.84%, and the between-run precision was 0.68%-3.38% for Reflotron-Hemoglobin dry-chemistry system, respectively. The linearity range was 52-191 g/L . The results of Reflotron-Hemoglobin dry-chemistry system were correlated well with those of Beckman-Coulter LH750 system, and the correlation equation wan Y=1.031 6X-2.796 9. The verification coincidence rate for reference range was 100%. Conclusions The within-run and between-run precisions of Reflotron-Hemoglobin dry-chemistry system for the determination of hemoglobin are good with accurate results for both normal and abnormal samples. The reference range can be used in this system.

Key words: Hemoglobin, Performance evaluation, Precision, Linearity

CLC Number:

R446.11

CHEN Liting, WANG Jianbiao, LIN Xiaoyi, SHI Hourong.. Evaluation on the performance of Reflotron-Hemoglobin dry-chemistry system[J]. , 2014, 29(5): 518-520.

[1]	WANG Wei, ZHANG Zhixin, ZHANG Chuanbao, ZHOU Weiyan, WANG Zhiguo. Establishment of allowable total error，allowable imprecision and allowable bias for 22 endocrine analytes [J]. Laboratory Medicine, 2023, 38(8): 707-712.
[2]	TAN Chunyan, ZHOU Ying, MO Zhijiang. Errors in Appendix B examples of WS/T 505—2017 and correction [J]. Laboratory Medicine, 2023, 38(6): 569-573.
[3]	YANG Xiaodong, LI Quanle, PAN Qingqing, ZHOU Jing, XIE Yangmin, ZOU Jihua, SHEN Min, ZHANG Man. Establishment and performance evaluation of candidate reference measurement procedure for the determination of immunosuppressive drugs in human whole blood by ID-LC-MS/MS [J]. Laboratory Medicine, 2023, 38(3): 215-222.
[4]	ZHENG Zhiyou, LI Xingzhao, XIE Yage. Changes and clinical significance of HbA_1c，NT-proBNP，Hcy，SOD and TC levels in patients with diabetes cardiomyopathy [J]. Laboratory Medicine, 2023, 38(3): 282-286.
[5]	XU Qianqian, JIANG Wangqing, SHEN Linjie, LU Yingjie, CHEN Jian, JIANG Haoqin. Establishment of the reference intervals for related parameters of adult reticulocytes in Shanghai [J]. Laboratory Medicine, 2022, 37(7): 641-645.
[6]	GAO Feng. Research progress of new tumor molecular markers in the age of precision medicine：from accurate diagnosis to precision chemotherapy [J]. Laboratory Medicine, 2022, 37(4): 309-312.
[7]	HONG Jun, RAO Yongcai. Establishment and evaluation of CD157-based 4-color PNH assay for neutrophil and monocyte by flow cytometry [J]. Laboratory Medicine, 2022, 37(4): 377-381.
[8]	LI Xiaobo, PU Zhifei, WU Xiaoqi, LIU Yangyang, XIE Xuanbo, SHAN Hanming, YU Jiaping. Application of CLSI EP06-Ed2 in the linear verification of quantitative measurement procedures [J]. Laboratory Medicine, 2022, 37(3): 230-234.
[9]	LIU Congcong, LI Jiaping, ZHOU Hongwei. Performance evaluation of 2 mass spectrometry systems in clinical microbial identification [J]. Laboratory Medicine, 2022, 37(11): 1084-1088.
[10]	QU Yuanqing, ZHAO Fan, YE Ailian, LI Lifeng, ZHAO Haitao, LI Huizhen, LIANG Zhuohui, LI Liang. Relationship between plasma omentin-1 level and metabolic clearance rate in type 2 diabetes mellitus patients [J]. Laboratory Medicine, 2022, 37(1): 28-31.
[11]	YUE Daoyuan, CHEN Wei, LIAO Qing, YU Weiting, LI Huijun. Establishment and evaluation of Wuhan Glycohemoglobin Harmonization Program [J]. Laboratory Medicine, 2022, 37(1): 78-82.
[12]	CHEN Yujia, SUN Zhiyong, WANG Shaojing, YANG Jiangtao, WU Benqing. Application of self-made drying device in the preparation of dried blood filter paper samples [J]. Laboratory Medicine, 2021, 36(8): 857-863.
[13]	WANG Wei, WANG Lei, WANG Beili, ZHOU Fangfang, HU Jun, WU Pei, YIN Fen, YUAN Kaiying, HOU Yumin, DIAO Duanqin, LI Guoying, CHEN Min, GAO Yuhong. Establishment and preliminary evaluation of HbA_1c determination harmonization plan of Xuhui District of Shanghai [J]. Laboratory Medicine, 2021, 36(5): 549-553.
[14]	TIAN Yueru, WANG Jingjing, AI Fuqi, MA Yimin, WANG Bei, JIANG Xiaofei. Performance evaluation of Vitek 2 Compact AST-N334,AST-N335,AST-P639 Chinese antimicrobial susceptibility test cards [J]. Laboratory Medicine, 2021, 36(4): 417-423.
[15]	Clinical Diagnosis and Laboratory Medicine Branch of China Maternal and Child Health Association. Expert consensus on establishment of standards for the performance evaluation of systems for whole blood C-reactive protein determination of Chinese children [J]. Laboratory Medicine, 2021, 36(12): 1201-1205.

Evaluation on the performance of Reflotron-Hemoglobin dry-chemistry system

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics