Abstract: Objective To investigate the rare Diego(Di) and Dombrock(Do) blood group systems of 200 random blood donors of Han nationality in Jiangxi province, and to obtain the appropriate gene frequency data for clinical blood transfusion. Methods A total of 200 random blood donors of Han nationality in Jiangxi province were enrolled, and polymerase chain reaction-sequence specific primer (PCR-SSP) was used to detect Di and Do blood group systems. Results In the 200 cases, there were 2 types of gene phenotypes of Di blood group, Di^a-b+(196 cases) and Di^a+b+(4 cases). Di^a+b-and Di^a-b- were not detected. The gene frequency of Di^a was 0.01, and the gene frequency of Di^b was 0.99.Do blood group identified 3 gene phenotypes, Do^a+b- (2 cases), Do^a+b+ (16 cases) and Do^a-b+ (182 cases). The gene frequency of Do^a was 0.05, and the gene frequency of Do^b was 0.95. Results The gene phenotypes of Di blood group were Di^a-b+ mainly and Di^a+b+, and Di^a+b- and Di^a-b- were not detected. The gene phenotypes of Do blood group were Do^a-b+ mainly, Do^a+b+ and Do^a+b-.

Key words: Diego blood group, Dombrock blood group, Polymerase chain reaction-sequence specific primer