Abstract:

Objective　 To detect and diagnose respiratory pathogens in the monitoring specimens of avian influenza A (H7N9) virus. Methods　 Nucleic acids of 22 kinds of respiratory pathogens were determined by multiple reverse transcription polymerase chain reaction (RT-PCR). Relative respiratory pathogens were isolated and indentified. Results 　Among the monitoring specimens of avian influenza A (H7N9) virus, nucleic acid of respiratory syncytial virus A (RSV-A) was determined from a severe pneumonia illness patient. The specimen was isolated by MRC-5, and 1 strain of RSV-A was isolated successfully. Conclusions 　Multiple RT-PCR could test 22 kinds of respiratory pathogens simultaneously and contributes to diagnose and control the epidemic situation. The isolation of RSV-A would provide a foothold for the further research of respiratory syncytial virus.

Key words: Avian influenza A (H7N9) virus, Respiratory syncytial virus, Human infection, Multiple reverse transcription polymerase chain reaction