Abstract:

Objective To evaluate the application significance of UF-1000i urine formed element analyzer in the screening of urinary tract infection(UTI). Methods Medistream urine samples were collected from 148 patients with suspected UTI. Bacterium (BACT) counts and white blood cell (WBC) counts were determined by UF-1000i urine formed element analyzer. By comparing with quantitative urine culture as the golden standard of UTI,the positive cut-off criteria for UF-1000i was set through receiver operating characteristic (ROC) curve. The sensitivity,specificity,positive predictive value,negative predictive value and accuracy were evaluated. Results The cut-off values for BACT and WBC counts were 325/μL and 48/μL,respectively. The sensitivity,specificity,positive predictive value,negative predictive value and accuracy of BACT and WBC counts were 78.3%,92.2%,81.8%,90.4%,87.8% and 73.9%,81.4%,64.2%,87.4%,79.1%, respectively. The combined determination of BACT and WBC counts by UF-1000i showed that the sensitivity, specificity, positive and negative predictive values and accuracy were 95.7%,70.6%,59.5%,97.3% and 78.4%, respectively. Conclusions UF-1000i urine formed element analyzer may be a screening tool for UTI.

Key words: Urinary tract infection, Urinalysis, UF-1000i analyzer