Separation and purification of HbA1c and HbA0

doi:10.3969/j.issn.1673-8640.2013.10.010

Abstract

Abstract:

Objective To separate and purify hemoglobin A_1c(HbA_1c) and hemoglobin A₀(HbA₀) from red blood cells of healthy subjects. Methods Collecting whole blood from healthy subjects, the red blood cells were separated and prepared for hemolysis. HbA_1c and HbA₀ were separated by weak acid positive ion exchange chromatography and purified by boric acid agarose affinity chromatography respectively. Results Hand-loaded column with weak acid positive ion exchange chromatography was used to separate and collect the peaks of HbA_1c and HbA₀ from healthy subjects′ red blood cell hemolysis, and the purities were 79.78% and 97.42% by high performance liquid chromatography (HPLC) respectively. After being purified by boric acid agarose affinity chromatography,the final purities of HbA_1c and HbA₀ were 94.3% and 99.7% respectively. Conclusions The single ion exchange chromatography for separating the glycosylated hemoglobin is not ideal,and it must be combined with affinity chromatography to obtain a high purities of HbA_1c and HbA₀.

Key words: Hemoglobin A_1c, Hemoglobin A₀, Ion exchange resin, Affinity chromatography

CLC Number:

R446.1

TU Guohua,WANG Bijun,ZHU Yunjiao,QIN Ying,JIANG Xugan.. Separation and purification of HbA_1c and HbA₀[J]. , 2013, 28(10): 904-907.

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Separation and purification of HbA_1c and HbA₀

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