Comparison study on the determination of colony morphology of Ureaplasma urealyticum by two kinds of culture media

doi:10.3969/j.issn.1673-8640.2013.05.004

Abstract

Abstract: Objective The results of detecting Ureaplasma urealyticum(Uu) from different kinds of samples in the liquid medium and the selective solid medium were compared, and morphological characters of Uu colonies by the selective solid culture medium were observed. Methods The 1698 samples from urogenital tract were cultured in the liquid medium and the selective solid medium. The positive rates from the two assays and three kinds of samples were compared and analyzed. The colonies features of Uu were observed by the selective solid medium. Results The positive rate of Uu from female urogenital tract was significantly（49.9%）higher than that from semen（39.6%）and urethral discharge（19.2%）（P<0.05）. There was significant difference of the positive rate from female urogenital tract between the liquid medium and the selective solid medium（P<0.05）. However, there were no statistics significance of the positive rate from male urogenital tract between the two assays（P>0.05）. The main colonies of Uu was typical echinus colony(77.5%), fried egg colony（13.8%）, vesicle colony, tiny colony, coryneform colony and mixed colony. Conclusions The positive rate of Uu from female urogenital tract is higher than that from semen samples, and the positive rate from male urethral discharge is the lowest. The results of Uu from female specimens using two media are different obviously. There is a good consistency between the two ways about the male samples. Uu colonies have morphological heterogeneity.

Key words: Solid medium culture, Liquid medium culture, Colony, Ureaplasma urealyticum

ZHOU Yunheng 1，MA Hongxia 2， CAO Guangya 1，SHI Xiaoxing 1，LIU Fenghua 1. Comparison study on the determination of colony morphology of Ureaplasma urealyticum by two kinds of culture media[J]. , 2013, 28(5): 362-365.

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