质谱技术在临床实验室的应用

doi:10.3969/j.issn.1673-8640.2019.01.001

摘要/Abstract

摘要：

质谱技术较传统检测方法具有独特优势,在临床应用中展现出巨大的实用性及潜力。已有的质谱技术可大致归入3类仪器平台——三重四极杆质谱、飞行时间质谱（TOF-MS）、电感耦合等离子体质谱（ICP-MS）。文章对临床实验室基于质谱技术所开展的检测项目作一介绍,着重就相应技术如何满足临床需求进行阐述,并介绍临床实验室质谱技术的新兴应用领域,希望帮助读者了解质谱技术在医疗领域的应用情况,以便更好地为患者服务。

关键词: 质谱技术, 三重四级杆质谱, 飞行时间质谱, 电感耦合等离子体质谱, 临床实验室, 检测项目

Abstract:

Mass spectrometry has demonstrated great utility and potential in clinical applications due to its unique advantages over traditional methods. The current applications are categorized into 3 different instrument platforms and technologies,including tripe quadruple mass spectrometry,time-of-flight mass spectrometry（TOF-MS） and inductively coupled plasma mass spectrometry（ICP-MS）. This review is intended to provide an overview of determination items based on mass spectrometry in clinical laboratories and to focus on how technologies best support clinical needs. Furthermore,this review discusses the emerging applications of mass spectrometry in clinical laboratories to provide insights into the application of mass spectrometry in healthcare to better serve patient needs in the near future.

Key words: Mass spectrometry, Tripe quadruple mass spectrometry, Time-of-flight mass spectrometry, Inductively coupled plasma mass spectrometry, Clinical Laboratory, Determination item

中图分类号:

Q503

Yan Victoria Zhang, Thomas Jackson. 质谱技术在临床实验室的应用[J]. 检验医学, 2019, 34(1): 1-7.

Yan Victoria Zhang, Thomas Jackson. Clinical mass spectrometry：applications in clinical laboratories[J]. Laboratory Medicine, 2019, 34(1): 1-7.

参考文献 41

[1]	COUCHMAN L,MONIZ C F.Analytical considerations for the biochemical assessment of vitamin D status[J]. Ther Adv Musculoskeletal Dis,2017,9(4):97-104.
[2]	LE GOFF C,SOUBERBIELLE J C,DELVIN E,et al.Vitamin D measurement: pre-analytical and analytical considerations[J]. Ann Biol Clini(Paris),2015,73(1):79-92.
[3]	CECCATO F,BOSCARO M.Cushing's syndrome: screening and diagnosis[J]. High Blood Press Cardiovasc Prev,2016,23(3):209-215.
[4]	HIRSH S,BEN-DOR A.Finding a needle in a haystack: the advantages of liquid chromatography-tandem mass spectrometry (LC-MS/MS) in determination of sex hormones in children[J]. Pediatr Endocrinol Rev,2016,13(4):714-719.
[5]	WANG Q, MESAROS C,BLAIR I A,et al.Ultra-high sensitivity analysis of estrogens for special populations in serum and plasma by liquid chromatography-mass spectrometry:assay considerations and suggested practices[J]. J Steroid Biochem Mol Biol,2016,162:70-79.
[6]	AUCELLA F,LAURIOLA V,VECCHIONE G,et al.Liquid chromatography-tandem mass spectrometry method as the golden standard for therapeutic drug monitoring in renal transplant[J]. J Pharm Biomed Anal,2013,86:123-126.
[7]	GHAREEB M,AKHLAGHI F.Alternative matrices for therapeutic drug monitoring of immunosuppressive agents using LC-MS/MS[J]. Bioanalysis,2015,7(8):1037-1058.
[8]	EZGU F.Inborn errors of metabolism[J]. Adv Clin Chem,2016,73:195-250.
[9]	OMBRONE D,GIOCALIERE E,FORNI G,et al.Expanded newborn screening by mass spectrometry:new tests,future perspectives[J]. Mass Spectrom Rev,2016,35(1):71-84.
[10]	ANGELETTI S.Matrix assisted laser desorption time of flight mass spectrometry (MALDI-TOF MS) in clinical microbiology[J]. J Microbiol Methods,2017,138:20-29.
[11]	CHENG K,CHUI H,DOMISH L,et al.Recent development of mass spectrometry and proteomics applications in identification and typing of bacteria[J].Proteomics Clin Appl,2016,10(4):346-357.
[12]	SHEN Y,TOLI N,PIEHOWSKI P D,et al.High-resolution ultrahigh-pressure long column reversed-phase liquid chromatography for top-down proteomics[J]. J Chromatogr A, 2017,1498:99-110.
[13]	SHI T,SONG E,NIE S,et al.Advances in targeted proteomics and applications to biomedical research[J]. Proteomics,2016,16(15-16):2160-2182.
[14]	BODEAU-LIVINEC F,GLORENNEC P,COT M,et al. Elevated blood lead levels in infants and mothers in benin and potential sources of exposure[J]. Int J Environ Res Public Health,2016,13(3):pii:E316.
[15]	PEREZ A L,NEMBHARD M,MONNOT A,et al.Child and adult exposure and health risk evaluation following the use of metal- and metalloid-containing costume cosmetics sold in the United States[J]. Regul Toxicol Pharmacol,2017,84:54-63.
[16]	MARCINKOWSKA M,BARAŁKIEWICZ D. Multielemental speciation analysis by advanced hyphenated technique-HPLC/ICP-MS:a review[J]. Talanta,2016,161:177-204.
[17]	唐古生,吴豫,沈茜. 免疫检测干扰因素的分析、识别和对策[J]. 中华检验医学杂志,2009,32(7):725-729.
[18]	CHACE D H,KALAS T A.A biochemical perspective on the use of tandem mass spectrometry for newborn screening and clinical testing[J]. Clin Biochem,2005,38(4):296-309.
[19]	CHEN Y,YAZDANPANAH M,HOFFMAN B R,et al.Rapid determination of serum testosterone by liquid chromatography-isotope dilution tandem mass spectrometry and a split sample comparison with three automated immunoassays[J]. Clin Biochem,2009,42(6):484-490.
[20]	HARING R,BAUMEISTER S E,NAUCK M,et al.Testosterone and cardiometabolic risk in the general population-the impact of measurement method on risk associations: a comparative study between immunoassay and mass spectrometry[J]. Eur J Endocrinol,2013,169(4):463-470.
[21]	FRENCH D.Advances in bioanalytical techniques to measure steroid hormones in serum[J]. Bioanalysis,2016,8(11):1203-1219.
[22]	KEEVIL B G.LC-MS/MS analysis of steroids in the clinical laboratory[J]. Clin Biochem, 2016,49(13-14):989-997.
[23]	BEDNER M,LIPPA K A,TAI S S.An assessment of 25-hydroxyvitamin D measurements in comparability studies conducted by the Vitamin D Metabolites Quality Assurance Program[J]. Clin Chim Acta,2013,426:6-11.
[24]	ENKO D,FRIDRICH L,REZANKA E,et al.25-Hydroxy-vitamin D status:limitations in comparison and clinical interpretation of serum-levels across different assay methods[J]. Clin Lab,2014,60(9):1541-1550.
[25]	MOREAU E,BÄCHER S,MERY S,et al. Performance characteristics of the VIDAS® 25-OH vitamin D total assay-comparison with four immunoassays and two liquid chromatography-tandem mass spectrometry methods in a multicentric study[J]. Clin Chem Lab Med,2016,54(1):45-53.
[26]	SPANAUS K,VON ECKARDSTEIN A.Evaluation of two fully automated immunoassay based tests for the measurement of 1α,25-dihydroxyvitamin D in human serum and comparison with LC-MS/MS[J]. Clin Chem Lab Med,2017,55(9):1305-1314.
[27]	VOLMER D A,MENDES L R,STOKES C S.Analysis of vitamin D metabolic markers by mass spectrometry:current techniques,limitations of the "gold standard" method,and anticipated future directions[J]. Mass Spectrom Rev, 2015,34(1):2-23.
[28]	TAYLOR P J.Therapeutic drug monitoring of immunosuppressant drugs by high-performance liquid chromatography-mass spectrometry[J]. Ther Drug Monit,2004,26(2):215-219.
[29]	TAYLOR P J,TAI C H,FRANKLIN M E,et al.The current role of liquid chromatography-tandem mass spectrometry in therapeutic drug monitoring of immunosuppressant and antiretroviral drugs[J]. Clin Biochem,2011,44(1):14-20.
[30]	DASGUPTA A.Impact of interferences including metabolite crossreactivity on therapeutic drug monitoring results[J]. Ther Drug Monit, 2012,34(5):496-506.
[31]	SOLDIN S J,STEELE B W,WITTE D L,et al.Lack of specificity of cyclosporine immunoassays. Results of a College of American Pathologists Study[J]. Arch Pathol Lab Med,2003,127(1):19-22.
[32]	DELAFIORI J,RING G,FUREY A.Clinical applications of HPLC-ICP-MS element speciation:a review[J]. Talanta,2016,153:306-331.
[33]	NETZEL B C,GRANT R P,HOOFNAGLE A N,et al.First steps toward harmonization of LC-MS/MS thyroglobulin assays[J]. Clin Chem,2016,62(1):297-299.
[34]	ANTUNES M V,CHARÃO M F,LINDEN R. Dried blood spots analysis with mass spectrometry:potentials and pitfalls in therapeutic drug monitoring[J]. Clin Biochem,2016,49(13-14):1035-1046.
[35]	WAGNER M,TONOLI D,VARESIO E,et al.The use of mass spectrometry to analyze dried blood spots[J]. Mass Spectrom Rev,2016,35(3):361-438.
[36]	BAKER T C,HAN J,BORCHERS C H.Recent advancements in matrix-assisted laser desorption/ionization mass spectrometry imaging[J]. Curr Opin Biotechnol,2017,43:62-69.
[37]	BADU-TAWIAH A K,EBERLIN L S,OUYANG Z
	et al. Chemical aspects of the extractive methods of ambient ionization mass spectrometry[J]. Annu Rev Phys Chem,2013,64:481-505.
[38]	FLINDERS B,CUYPERS E,ZEIJLEMAKER H
	et al. Preparation of longitudinal sections of hair samples for the analysis of cocaine by MALDI-MS/MS and TOF-SIMS imaging[J]. Drug Test Anal,2015,7(10):859-865.
[39]	ANNESLEY T M,MCKEOWN D A,HOLT D W,et al.Standardization of LC-MS for therapeutic drug monitoring of tacrolimus[J]. Clin Chem,2013,59(11):1630-1637.
[40]	JOHNSON-DAVIS K L,JUENKE J M,THOMAS R L.et al. Everolimus method comparison between Waters MassTrakTM Immunosuppressants XE (IUO) kit and an in-house laboratory developed LC-MS/MS method in renal transplant patients[J]. Ann Clin Lab Sci,2015,45(1):27-31.
[41]	FERREIRA C R,YANNELL K E,JARMUSCH A K,et al.Ambient ionization mass spectrometry for point-of-care diagnostics and other clinical measurements[J]. Clin Chem,2016,62(1):99-110.