2型糖尿病患者CR1基因多态性与红细胞CR1数量、黏附活性相关性研究

检验医学 ›› 2012, Vol. 27 ›› Issue (9): 741-744.

2型糖尿病患者CR1基因多态性与红细胞CR1数量、黏附活性相关性研究

安新,高利常,王冬梅,邹吉敏,李超,袁宝军

河北联合大学附属开滦医院检验科，河北唐山 063000

收稿日期:2012-07-15 修回日期:2012-05-08 出版日期:2012-09-12 发布日期:2012-09-12
作者简介:安新，男，1963年生，学士，副主任技师，主要从事细胞免疫学及肝胆病学、风湿免疫病学研究。

Study on the correlation of CR1 gene polymorphism with erythrocyte CR1 expression and its adhesive activity in patients with type 2 diabetes mellitus

Department of Clinical Laboratory，Kailuan Affiliated Hospital of Hebei United University，Hebei Tangshan 063000，China

Received:2012-07-15 Revised:2012-05-08 Online:2012-09-12 Published:2012-09-12

摘要/Abstract

摘要： 目的　研究2型糖尿病（T2DM）患者1型补体受体（CR1）基因多态性与红细胞CR1数量、黏附活性的相关性。方法　应用限制性内切酶Hind Ⅲ聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术测定132例T2DM患者和124名正常健康人群CR1基因多态性，用酶联免疫吸附试验(ELISA)测定红细胞的CR1数量，检测红细胞黏附活性，并做统计学分析。结果　T2DM患者红细胞表面的CR1数量和黏附活性均低于正常对照组（P<0.01）。CR1基因型在2组的分布频率均为HH型最高、HL型次之、LL型最低。T2DM组CR1基因等位基因L频率为14.8%，高于对照组（11.3%），但2组CR1基因型频率和等位基因频率差异均无统计学意义（P>0.05）。2组组内比较，HH型与HL型红细胞CR1数量及黏附活性差异均无统计学意义（Ρ均>0.05）。T2DM组HH型与HL型红细胞CR1数量与黏附活性均低于正常对照组（Ρ<0.05）。结论　T2DM患者与正常对照者间CR1基因型频率和等位基因频率无差异，而T2DM患者红细胞CR1数量及黏附活性减低，提示T2DM的发生与红细胞免疫功能低下有关。

关键词: 1型补体受体, 基因多态性, 红细胞, 黏附活性, 2型糖尿病

Abstract: Objective　To study on the correlation of complement receptor type 1 (CR1) gene polymorphism with erythrocyte CR1 expression and its adhesive activity in patients with type 2 diabetes mellitus (T2DM). Methods　A total of 132 patients with T2DM were determined for the CR1 gene polymorphism by restriction incision enzyme Hind Ⅲ polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)，and enzyme-linked immunosorbent assay (ELISA) was performed to detect the expression of CR1 on erythrocyte and its adhesive activity.Meanwhile，124 healthy subjects were enrolled as controls. The results were analyzed statistically. Results　The expression of CR1 on erythrocyte and its adhesive activity in patients with T2DM were lower than those in healthy controls (P<0.01). The CR1 genotype distribution frequencies from high to low were type HH，type HL and type LL by turns. The allele L frequency of CR1 gene in T2DM patients (14.8%) was higher than that in healthy controls (11.3%)，and there was no statistical significance in CR1 genotype frequency and allele frequency between the 2 groups (P>0.05). The expression of CR1 and adhesive activity of type HH and type HL had no statistical significance (P>0.05). The expression of CR1 and adhesive activity of type HH and type HL in T2DM patients were significantly lower than those in healthy controls (P<0.05). Conclusions　The genotype and allele frequencies in patients with T2DM have no statistical difference with those in healthy controls，but the expression of CR1 on erythrocyte and the adhesive activity in patients with T2DM decrease significantly. It indicates that the decrease of erythrocyte CR1 adhesive activity may be attribute to the occurrence of T2DM.

Key words: Complement receptor 1, Gene polymorphism, Erythrocyte, Adhesive activity, Type 2 diabetes mellitus

安　新;高利常;王冬梅;邹吉敏;李　超;袁宝军. 2型糖尿病患者CR1基因多态性与红细胞CR1数量、黏附活性相关性研究[J]. 检验医学, 2012, 27(9): 741-744.

AN Xin;GAO Lichang;WANG Dongmei;ZOU Jimin;LI Chao;YUAN Baojun. Study on the correlation of CR1 gene polymorphism with erythrocyte CR1 expression and its adhesive activity in patients with type 2 diabetes mellitus[J]. , 2012, 27(9): 741-744.

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