耐碳青霉烯类鲍曼不动杆菌6种基因及ISAbal插入序列的研究

检验医学 ›› 2012, Vol. 27 ›› Issue (9): 749-753.

耐碳青霉烯类鲍曼不动杆菌6种基因及ISAbal插入序列的研究

廖晚珍

1. 南昌大学第一附属医院检验科，江西南昌 330006；2. 南昌大学第一附属医院呼吸科，江西南昌 330006

收稿日期:2012-07-03 修回日期:2012-08-31 出版日期:2012-09-12 发布日期:2012-09-12
通讯作者: 廖晚珍
作者简介:廖晚珍，男，1952年生，副主任技师，主要从事临床细菌学与真菌学、分子生物学研究。

Research on six genes and insertion sequence ISAbal of carbopenems-resistant Acinetobacter baumannii

1. Department of Clinical Laboratory，the First Affiliated Hospital of Nanchang University，Jiangxi Nanchang 330006，China; 2. Department of Respiration，the First Affiliated Hospital of Nanchang University，Jiangxi Nanchang 330006，China

Received:2012-07-03 Revised:2012-08-31 Online:2012-09-12 Published:2012-09-12

摘要/Abstract

摘要： 目的　研究耐碳青霉烯类鲍曼不动杆菌（CRAB）的基因型特点和与插入序列ISAbal的关系，探讨其耐药机制。方法　收集CRAB 70株（非重复）和碳青霉烯类敏感鲍曼不动杆菌10株，采用2-巯基丙酸抑制试验检测金属β-内酰胺酶（MBLs）；采用多重聚合酶链反应(PCR)扩增bla_IMP、bla_VIM、bla_OXA-23、bla_OXA-24、bla_OXA-51、bla_OXA-58、ISAbal_OXA-23，扩增产物DNA测序进行比对。结果　70株CRAB 2-巯基丙酸与头孢他啶协同试验全部为阴性（即MBLs阴性），用7对特异性引物对70株CRAB和10株敏感菌进行PCR扩增，所有CRAB均携带bla_OXA-23、bla_OXA-51，未检出bla_OXA-24；1株含有bla_OXA-58；80株均不携带bla_IMP和bla_VIM；插入序列ISAbal_OXA-23 70株CRAB均有表达，敏感菌均不表达。结论　CRAB的主要耐药基因是bla_OXA-51和bla_OXA-23；另外，bla_OXA-23上游都存在插入序列ISAbal。鲍曼不动杆菌的耐药性与MBLs、bla_OXA-24、bla_OXA-58无相关性。

关键词:

Abstract: Objective　To study the relationship between the genotype characteristics and insertion sequence ISAbal of carbopenems-resistant Acinetobacter baumannii(CRAB)，and investigate the drug-resistant mechanism. Methods　A total of 70 strains of CRAB (no repeat strains) and 10 strains of carbopenems-sensitive Acinetobacter baumannii were collected. Metalloenzyme beta-lactamase (MBLs) were detected by 2-mercaptopropionic acid inhibition test. The bla_IMP，bla_VIM，bla_OXA-23，bla_OXA-24，bla_OXA-51，bla_OXA-58 and ISAbal_OXA-23 were amplified by multiplex polymerase chain reaction (PCR). The amplified products were measured for DNA sequence. Results　The synergy tests were negative (MBLs negative) by 2-mercaptopropionic acid and ceftazime in 70 strains of CRAB. Through 7 pairs of specific primers，70 strains of CRAB and 10 strains of carbopenems-sensitive Acinetobacter baumannii were determined for PCR amplification. All the CRAB strains carried bla_OXA-23 and bla_OXA-51，but bla_OXA-24 was never detected. Only 1 strain carried bla_OXA-58，and the 80 strains did not carry bla_IMPand bla_VIM. In addition，all the CRAB strains carried insertion sequence ISAbal_OXA-23，however，all the sensitive strains did not carry. Conclusions　Major genes are bla_OXA-51 and bla_OXA-23 in the CRAB strains. Insertion sequence ISAbal is located in the upstream of bla_OXA-23. There is no correlation among drug-resistance of Acinetobacter baumannii to MBLs，bla_OXA-24 and bla_OXA-58.

廖晚珍;杨　璐;温桂兰;彭卫华;胡雪飞;余　阳. 耐碳青霉烯类鲍曼不动杆菌6种基因及ISAbal插入序列的研究[J]. 检验医学, 2012, 27(9): 749-753.

LIAO Wanzhen;YANG Lu;WEN Guilan;PENG Weihua;HU Xuefei;YU Yang . Research on six genes and insertion sequence ISAbal of carbopenems-resistant Acinetobacter baumannii[J]. , 2012, 27(9): 749-753.