关键词: 微小RNA-145-5p, 微小RNA-200C-3p, 微小RNA-1290, 甲状腺乳头状癌, 复发

Abstract:

Objective To investigate the roles of serum miR-145-5p，miR-200C-3p and miR-1290 expressions in the assessment of recurrence in patients with papillary thyroid carcinoma（PTC）. Methods A total of 93 patients with PTC（PTC group），91 patients with thyroid nodules（benign nodule group） and 94 healthy subjects（healthy control group） from Liyang People's Hospital were enrolled. The clinical data were collected，and the relative expression levels of serum miR-145-5p，miR-200C-3p and miR-1290 were determined. The patients with PTC were followed up for 2 years，and the recurrence situation was recorded. The patients were classified into non-recurrence group（73 cases） and recurrence group（20 cases）. Receiver operating characteristic（ROC） curve was used to evaluate the efficacy of serum miR-145-5p，miR-200C-3p and miR-1290 relative expression levels in predicting recurrence in PTC patients. Kaplan-Meier survival curve was used to analyze the recurrence situation of PTC patients. Results The relative expression level of serum miR-145-5p in PTC group was lower than those in benign nodule group and healthy control group（P<0.05），and the relative expression levels of miR-200C-3p and miR-1290 were higher than those in benign nodule group and healthy control group（P<0.05）. The relative expression level of serum miR-145-5p in benign nodule group was lower than that in healthy control group（P<0.05），and the relative expression levels of miR-200C-3p and miR-1290 were higher than those in healthy control group（P<0.05）. The relative expression level of serum miR-145-5p in patients with lymphatic metastasis was lower than that in patients without lymphatic metastasis（P<0.001），and the relative expression levels of miR-200C-3p and miR-1290 were higher than those in patients without lymphatic metastasis（P<0.001）. There was no statistical significance in the relative expression levels of serum miR-145-5p，miR-200C-3p and miR-1290 among patients with different genders，ages，maximum tumor diameters，TNM stages and vascular space infiltration（P>0.05）. The relative expression level of serum miR-145-5p in recurrence group was lower than that in non-recurrence group（P<0.001），and the relative expression levels of miR-200C-3p and miR-1290 were higher than those in non-recurrence group（P<0.001）. The areas under curves（AUC） of single and combined determinations of serum miR-145-5p， miR-200C-3p and miR-1290 for predicting recurrence in PTC patients were 0.805，0.842，0.861 and 0.942，respectively. The recurrence rate in miR-145-5p low-expression group was higher than that in miR-145-5p high-expression group（P=0.011），and the recurrence rates in miR-200C-3p and miR-1290 low-expression groups were lower than those in miR-200C-3p and miR-1290 high-expression groups（P<0.001，P=0.001）. Conclusions Serum miR-145-5p in PTC patients is lowly expressed，while miR-200C-3p and miR-1290 are highly expressed，and they are related to recurrence，which may serve as biomarkers for prognosis assessment of PTC.

Key words: MicroRNA-145-5p, MicroRNA-200C-3p, MicroRNA-1290, Papillary thyroid carcinoma, Recurrence