口腔种植体周围炎患者血清干扰素调节因子4、可溶性致癌抑制因子2检测的临床意义

doi:10.3969/j.issn.1673-8640.2024.08.001

摘要/Abstract

摘要：

目的探讨口腔种植体周围炎患者血清干扰素调节因子4（IRF4）、可溶性致癌抑制因子2（sST2）检测的临床意义。方法选取2021年1月—2022年12月沧州市人民医院口腔种植体周围炎患者30例（口腔种植体周围炎组）、同一类型种植体的健康口腔种植体者30例（健康口腔种植体组）和体检健康者30名（正常对照组）。检测所有研究对象血清IRF4、sST2水平。采用Pearson相关分析评估血清IRF4、sST2水平与年龄、探诊深度（PD）、龈沟液量、出血指数（SBI）的相关性。采用受试者工作特征（ROC）曲线评估血清IRF4、sST2诊断口腔种植体周围炎的效能。结果口腔种植体周围炎组PD、龈沟液量、SBI均显著高于健康口腔种植体组和正常对照组（P<0.05）。正常对照组、健康口腔种植体组、口腔种植体周围炎组血清IRF4、sST2水平依次升高（P<0.001）。口腔种植体周围炎患者男性、女性之间血清IRF4、sST2水平差异均无统计学意义（P>0.05）。≥60岁的口腔种植体周围炎患者血清IRF4、sST2水平高于<60岁的患者（P<0.001）。血清IRF4、sST2与年龄、PD、龈沟液量、SBI均呈正相关（P<0.05）。血清IRF4、sST2单项检测和联合检测诊断口腔种植体周围炎的曲线下面积（AUC）分别为0.919、0.890、0.978。结论口腔种植体周围炎患者血清IRF4、sST2水平显著升高，与牙周指标密切相关，或可作为口腔种植体周围炎的诊断指标。

关键词: 干扰素调节因子4, 可溶性致癌抑制因子2, 口腔种植体周围炎

Abstract:

Objective To investigate the roles of serum interferon regulatory factor 4（IRF4） and soluble suppression of tumorigenicity 2（sST2） in patients with oral periimplantitis. Methods Totally，30 patients with oral periimplantitis treated in Cangzhou People's Hospital from January 2021 to December 2022（oral periimplantitis group） were enrolled，and another 30 healthy oral implant subjects（healthy oral implant group） and 30 healthy subjects（healthy control group） were enrolled. Serum levels of IRF4 and sST2 were compared among all the groups. The correlations of serum IRF4 and sST2 levels with age，periodontal depth（PD），gingival sulcus fluid volume and sulcus bleeding index（SBI） were analyzed by Pearson correlation analysis. Receiver operating characteristic（ROC） curve was used to analyze the predictive values of serum IRF4 and sST2 levels in oral periimplantitis. Results PD，gingival sulcus fluid volume and SBI in oral periimplantitis group were higher than those in healthy oral implant group and healthy control group（P<0.05）. Serum IRF4 and sST2 levels in healthy control group，healthy oral implant group and oral periimplantitis group were increased successively（P<0.001）. There was no statistical significance in serum IRF4 and sST2 levels between male and female patients with oral periimplantitis（P>0.05）. Serum IRF4 and sST2 levels in patients ≥60 years old were higher than those in patients <60 years old（P<0.001）. Serum IRF4 and sST2 were positively correlated with age，PD，gingival sulcus fluid volume and SBI（P<0.05）. The areas under curves（AUC） of serum IRF4 and sST2 single and combined determinations were 0.919，0.890 and 0.978，respectively. Conclusions Serum IRF4 and sST2 levels are increased in patients with oral periimplantitis，which are related to periodontal indexes，and they may be used in the diagnosis of oral periimplantitis.

Key words: Interferon regulatory factor 4, Soluble suppression of tumorigenicity 2, Oral periimplantitis

中图分类号:

R446.1

吕敏敏, 黄莹, 于兰, 曹丽婷, 曾翠敏. 口腔种植体周围炎患者血清干扰素调节因子4、可溶性致癌抑制因子2检测的临床意义[J]. 检验医学, 2024, 39(8): 723-727.

LÜ Minmin, HUANG Ying, YU Lan, CAO Liting, ZENG Cuimin. Determinations of serum interferon regulatory factor 4 and soluble suppression of tumorigenicity 2 in patients with oral periimplantitis[J]. Laboratory Medicine, 2024, 39(8): 723-727.

图/表 6

参考文献 21

[1]	DE AVILA E D, VAN OIRSCHOT B A, VAN DEN BEUCKEN J J J P. Biomaterial-based possibilities for managing peri-implantitis[J]. J Periodontal Res, 2020, 55(2):165-173. DOI PMID
[2]	ALVES C H, RUSSI K L, ROCHA N C, et al. Host-microbiome interactions regarding peri-implantitis and dental implant loss[J]. J Transl Med, 2022, 20(1):425. DOI PMID
[3]	左艳萍, 汪永跃, 王荣, 等. 挤压松质骨后植入口腔种植体的生物力学研究[J]. 实用临床医药杂志, 2021, 25(12):1-4.
[4]	ROKAYA D, SRIMANEEPONG V, WISITRASAMEEWON W, et al. Peri-implantitis update:risk indicators,diagnosis,and treatment[J]. Eur J Dent, 2020, 14(4):672-682.
[5]	WONG R W J, ONG J Z L, THEARDY M S, et al. IRF4 as an oncogenic master transcription factor[J]. Cancers(Basel), 2022, 14(17):4314.
[6]	BEETLER D J, BRUNO K A, DI FLORIO D N, et al. Sex and age differences in sST2 in cardiovascular disease[J]. Front Cardiovasc Med, 2023, 9:1073814.
[7]	伊韦尔, 吉奥凡诺利, 丁一. 口腔种植体周围炎[M]. 北京: 人民军医出版社, 2015.
[8]	KORMAS I, PEDERCINI C, PEDERCINI A, et al. Peri-implant diseases:diagnosis,clinical,histological,microbiological characteristics and treatment strategies. A narrative review[J]. Antibiotics(Basel), 2020, 9(11):835.
[9]	ROCCUZZO A, STÄHLI A, MONJE A, et al. Peri-implantitis:a clinical update on prevalence and surgical treatment outcomes[J]. J Clin Med, 2021, 10(5):1107.
[10]	柯秋蕾, 黄劲, 陈爱征, 等. GDM孕妇龈下菌群分布和牙周健康指数与牙周病的关系[J]. 检验医学, 2023, 38(2):163-166. DOI
[11]	LU J, LIANG T, LI P, et al. Regulatory effects of IRF4 on immune cells in the tumor microenvironment[J]. Front Immunol, 2023, 14:1086803.
[12]	ZHENG Y, ZHOU W, LI M, et al. IRF4-activated TEX41 promotes the malignant behaviors of melanoma cells by targeting miR-103a-3p/C1QB axis[J]. BMC Cancer, 2021, 21(1):1339. DOI PMID
[13]	RODRÍGUEZ-CARRIO J, LÓPEZ P, ALPERI-LÓPEZ M, et al. IRF4 and IRGs delineate clinically relevant gene expression signatures in systemic lupus erythematosus and rheumatoid arthritis[J]. Front Immunol, 2019, 9:3085.
[14]	WONG R W J, TAN T K, AMANDA S, et al. Feed-forward regulatory loop driven by IRF4 and NF-κB in adult T-cell leukemia/lymphoma[J]. Blood, 2020, 135(12):934-947. DOI PMID
[15]	HAN W, CHEN X, WANG X, et al. TLR-4,TLR-5 and IRF4 are diagnostic markers of knee osteoarthritis in the middle-aged and elderly patients and related to disease activity and inflammatory factors[J]. Exp Ther Med, 2020, 20(2):1291-1298.
[16]	张伟, 郭慧娟, 吴系美, 等. 血清sST2在类风湿性关节炎病情及疗效监测中的价值[J]. 检验医学, 2021, 36(12):1238-1242. DOI
[17]	ALEKSOVA A, PALDINO A, BELTRAMI A P, et al. Cardiac biomarkers in the emergency department:the role of soluble ST2(sST2) in acute heart failure and acute coronary syndrome-there is meat on the bone[J]. J Clin Med, 2019, 8(2):270.
[18]	PETRAMALA L, CONCISTRÈ A, SARLO F, et al. Assessment of sST2 behaviors to evaluate severity/clinical impact of acute pulmonary embolism[J]. Int J Mol Sci, 2023, 24(5):4591.
[19]	TOSCANO E C B, LESSA J M K, DE OLIVEIRA G N, et al. Peripheral levels of sST2 are increased in patients with temporal lobe epilepsy:additional evidence of low-grade inflammation[J]. Epilepsy Behav, 2020, 112:107351.
[20]	ZHANG Y, ZHU K, CHEN J, et al. Predictive values of serum IL-33 and sST2 in endotypes and postoperative recurrence of chronic rhinosinusitis with nasal polyps[J]. Mediators Inflamm, 2022, 2020:9155080.
[21]	TAKAHASHI M, TAKEDA M, MATSUMOTO S. Somatostatin enhances tooth-pulp-evoked cervical dorsal horn neuronal activity in the rat via inhibition of GABAergic interneurons[J]. Brain Res Bull, 2014, 100:76-83. DOI PMID

组别	例数	PD/mm	龈沟液量/μL	SBI	IRF4/（ng·mL^-1）	sST2/（ng·mL^-1）
正常对照组	30	2.52±0.80	0.68±0.23	0.63±0.19	4.21±1.32	28.05±3.64
健康口腔种植体组	30	2.58±0.84	0.87±0.26	0.75±0.26	5.15±1.67^*	41.63±5.28^*
口腔种植体周围炎组	30	4.62±1.36^*#	1.94±0.54^*#	3.31±1.05^*#	8.64±2.15^*#	52.76±6.94^*#
F值		40.257	100.774	168.002	53.566	154.361
P值		<0.001	<0.001	<0.001	<0.001	<0.001

组别	例数	PD/mm	龈沟液量/μL	SBI	IRF4/（ng·mL^-1）	sST2/（ng·mL^-1）
正常对照组	30	2.52±0.80	0.68±0.23	0.63±0.19	4.21±1.32	28.05±3.64
健康口腔种植体组	30	2.58±0.84	0.87±0.26	0.75±0.26	5.15±1.67^*	41.63±5.28^*
口腔种植体周围炎组	30	4.62±1.36^*#	1.94±0.54^*#	3.31±1.05^*#	8.64±2.15^*#	52.76±6.94^*#
F值		40.257	100.774	168.002	53.566	154.361
P值		<0.001	<0.001	<0.001	<0.001	<0.001

性别	例数	IRF4/（ng·mL^-1）	sST2/（ng·mL^-1）
男性	14	8.72±2.36	52.64±6.89
女性	16	8.57±2.13	52.87±7.03
t值		0.183	0.090
P值		0.856	0.929

性别	例数	IRF4/（ng·mL^-1）	sST2/（ng·mL^-1）
男性	14	8.72±2.36	52.64±6.89
女性	16	8.57±2.13	52.87±7.03
t值		0.183	0.090
P值		0.856	0.929

年龄	例数	IRF4/（ng·mL^-1）	sST2/（ng·mL^-1）
<60岁	19	6.58±2.14	46.75±5.82
≥60岁	11	12.20±3.25	63.14±8.46
t值		5.724	6.091
P值		<0.001	<0.001