TALENs技术在基因功能研究中的应用

doi:10.3969/j.issn.1673-8640.2014.05.002

检验医学 ›› 2014, Vol. 29 ›› Issue (5): 459-463.DOI: 10.3969/j.issn.1673-8640.2014.05.002

TALENs技术在基因功能研究中的应用

应春妹¹, 陈艺升², 郑冰²

1.复旦大学附属妇产科医院, 上海 200011;
2.上海交通大学医学院附属仁济医院, 上海 200127

收稿日期:2013-06-21 出版日期:2014-05-30 发布日期:2014-05-27
作者简介:应春妹, 女, 1967年生, 硕士, 主任技师, 主要从事临床微生物检验及细菌耐药性监测工作。

The application significance of TALEN technique for genome research

YING Chunmei¹, CHEN Yisheng², ZHEN Bing².

1.Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011, China;
2.Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China

Received:2013-06-21 Online:2014-05-30 Published:2014-05-27

摘要/Abstract

摘要：

基因敲除技术广泛应用于研究各类细胞和生物的基因功能。转录激活子样效应因子核酸酶技术(TALENs)是近年发展起来的一种新型基因敲除方法, 其利用TALEs蛋白核酸结合域氨基酸序列与其靶位点核酸序列之间存在着恒定对应关系, 从而组装出能特异性结合任意DNA序列的模块化蛋白。TALENs技术以其快速、高效的优势对生物基因研究领域产生深远影响, 为遗传疾病的治疗提供了新的策略。

关键词: 转录激活子样效应因子核酸酶技术, 基因敲除, 基因功能

Abstract:

Gene knockout is a broadly applicable approach for efficiently editing any gene of cells and organisms. The transcription activator-like effector nuclease (TALEN) technique is a newly-developed method, and it can target essentially any sequence by comprising a nonspecific DNA-cleaving nuclease fused to a DNA-binding domain, which can be engineered easily. TALEN technique with the capability to quickly and efficiently alter genes have profound impacts on biological research and get potential therapeutic strategies for genetic diseases.

Key words: Transcription activator-like effector nuclease technique, Gene knockout, Gene function

中图分类号:

Q503

应春妹, 陈艺升, 郑冰. TALENs技术在基因功能研究中的应用[J]. 检验医学, 2014, 29(5): 459-463.

YING Chunmei, CHEN Yisheng, ZHEN Bing.. The application significance of TALEN technique for genome research[J]. , 2014, 29(5): 459-463.

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TALENs技术在基因功能研究中的应用

The application significance of TALEN technique for genome research

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