LOX家族蛋白酶、弹性蛋白的mRNA表达与盆腔脏器脱垂的关系

检验医学 ›› 2014, Vol. 29 ›› Issue (12): 1218-1221.

LOX家族蛋白酶、弹性蛋白的mRNA表达与盆腔脏器脱垂的关系

吴慧恒¹, 李怀芳¹, 童晓文¹, 陈信良²

1.同济大学附属同济医院妇产科，上海 200065；2.上海交通大学医学院附属国际和平妇幼保健院妇科，上海 200030

收稿日期:2014-09-09 出版日期:2014-12-30 发布日期:2015-01-14
通讯作者: 陈信良,联系电话：021-64070434。
作者简介:吴慧恒,女,1990年生,硕士,主要从事盆腔脏器脱垂的分子生物学研究。
基金资助:
上海市卫生局科研课题（20124121）

Relationship on mRNA of lysyl oxidase and elastin with pelvic organ prolapse

WU Huiheng¹, LI Huaifang¹, TONG Xiaowen¹, CHEN Xinliang²

1.Department of Gynaecology and Obstetrics, Tongji Hospital, Tongji University, Shanghai 200065, China; 2. Department of Gynaecology, International Peace Maternity and Child Health Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200030, China

Received:2014-09-09 Online:2014-12-30 Published:2015-01-14

摘要/Abstract

摘要：

目的　探讨阴道前壁中赖氨酰氧化酶（LOX）家族和弹性蛋白的表达变化与盆腔脏器脱垂（POP）发生的关系。方法　选择因阴道壁膨出或子宫脱垂行全子宫切除术或阴道壁修补术患者39例，其中绝经前12例，绝经后27例；选择同期因妇科良性疾病、无压力性尿失禁或POP行全子宫切除术患者40例作为对照，其中绝经前27例，绝经后13例，采用实时荧光定量聚合酶链反应（PCR）检测患者阴道前壁组织中LOX家族蛋白酶和弹性蛋白mRNA的表达。结果　无论绝经与否，POP组患者阴道前壁中LOX、LOXL1、弹性蛋白mRNA的表达明显低于对照组（P＜0.05）；POP组中绝经后患者LOX、LOXL1、弹性蛋白 mRNA表达明显低于绝经前患者（P＜0.05）；阴道前壁中LOX、LOXL1 mRNA均与弹性蛋白mRNA呈直线正相关（r=0.936 1、0.689 8，P均＜0.05）。结论　阴道前壁组织中LOX、LOXL1表达下调可能是POP发生的一个原因。

关键词: 赖氨酰氧化酶, 弹性蛋白, 盆腔脏器脱垂

Abstract:

Objective　To investigate the expression of lysyl oxidase（LOX） and elastin in anterior vaginal wall of women with pelvic organ prolapse（POP） and the relationships with POP. Methods　The anterior vaginal wall samples were obtained from 39 POP patients undergoing anterior colporrhaphy or total hysterectomy， including 12 premenopausal cases due to vaginocele or uterine prolapse and 27 postmenopausal cases. The other anterior vaginal wall samples were obtained from 40 non-POP women（control group） undergoing total hysterectomy due to benign gynecological diseases， non-stress urinary incontinence or POP. Real-time fluorescence quantitation polymerase chain reaction（PCR） was used to verify the mRNA level of LOX and elastin. Results　The premenopausal and postmenopausal POP groups demonstrated significantly decreasing expressions of LOX， lysyl oxidase like 1（LOXL1） and elastin in anterior vaginal wall compared with control group（P＜0.05）.In POP group， the mRNA expressions of LOX， LOXL1 and elastin in postmenopausal patients were significantly lower than those in premenopausal patients（P＜0.05）. There was a positive correlation between LOX and LOXL1 mRNA with elastin mRNA in POP group（r=0.936 1 and 0.689 8， P＜0.05）. Conclusions　The decreasing expressions of LOX and LOXL1 in the anterior vaginal wall may contribute to POP.

Key words: Lysyl oxidase, Elastin, Pelvic organ prolapse

中图分类号:

R446.1

吴慧恒, 李怀芳, 童晓文, 陈信良. LOX家族蛋白酶、弹性蛋白的mRNA表达与盆腔脏器脱垂的关系[J]. 检验医学, 2014, 29(12): 1218-1221.

WU Huiheng, LI Huaifang, TONG Xiaowen, CHEN Xinliang. Relationship on mRNA of lysyl oxidase and elastin with pelvic organ prolapse[J]. , 2014, 29(12): 1218-1221.

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