抗精子抗体与精子形态的相关性调查

doi:10.3969/j.issn.1673-8640.2013.07.007

检验医学 ›› 2013, Vol. 28 ›› Issue (7): 590-594.DOI: 10.3969/j.issn.1673-8640.2013.07.007

抗精子抗体与精子形态的相关性调查

杨大干, 吕俊霞, 姚冬英, 沈乃仙

浙江大学医学院附属第一医院检验科, 浙江杭州 310003

收稿日期:2013-07-29 修回日期:2013-07-29 出版日期:2013-07-25 发布日期:2013-07-29
作者简介:杨大干,男,1975年生,硕士,副主任技师,主要从事临床基础检验工作。
基金资助:
浙江省人口和计划生育委员会科技项目(2010-KY-1-28)

Correlation investigation of anti-sperm antibodies and sperm morphology

YANG Dagan,Li Junxia,YAO Dongying, SHEN Naixian

Department of Clinical Laboratory, the First Affiliated Hospital, College of Medicine, Zhejiang University, Zhejiang Hangzhou 310003, China

Received:2013-07-29 Revised:2013-07-29 Online:2013-07-25 Published:2013-07-29

摘要/Abstract

摘要： 目的调查抗精子抗体(AsAb)对精子形态的影响。方法选取AsAb阳性组51例、疾病对照组40例和健康对照组47名,采用Diff-Quik染色法按世界卫生组织(WHO)精子形态分类标准进行精子形态评估,计算畸形精子指数(TZI)和精子畸形指数(SDI)。结果 AsAb阳性组的异常形态精子、锥形头、不定形头、颈中段增粗分别为80.2%±7.7%、6.3%±5.5%、11.0%±7.4%、21.9%±9.0%;疾病对照组分别为71.6%±5.8%、4.2%±4.5%、11.1%±4.8%、16.0%±6.9%;健康对照组分别为70.1%±6.4%、3.5%±4.6%、8.4%±4.3%、16.2%±6.9%;上述各指标在3组间差异均有统计学意义(F值分别为28.732、3.867、3.282、4.893,P<0.05或P＜0.01)。AsAb阳性组TZI和SDI分别为1.306±0.123、1.057±0.180;疾病对照组为1.255±0.096、0.901±0.119;健康对照组为1.257±0.098、0.882±0.121;3组间差异有统计学意义(F值分别为3.088、19.115,P＜0.05或P＜0.01)。结论 AsAb阳性可能是异常精子形态增多的原因之一,异常精子形态以锥形头、不定形头、颈中段增粗为主。

关键词: 抗精子抗体, 精子形态, 多重精子缺陷指数

Abstract: Objective To investigate the influence of anti-sperm antibodies (AsAb) on sperm morphology. Methods A total of 51 AsAb positive patients,40 control patients and 47 healthy controls were enrolled. The sperm morphology was evaluated according to World Health Organization (WHO) sperm morphology classification standards by Diff-Quik staining method. The abnormal sperm index (TZI) and sperm deformity index (SDI) were calculated. Results In the AsAb positive patients, the percentages of abnormal morphology (%), cone-shaped head (%), amorphous head (%) and middle neck thickening sperms (%) were 80.2?7.7, 6.3?5.5, 11.0?7.4 and 21.9?9.0, respectively. In the control patients, these percentages were 71.6?5.8, 4.2?4.5, 11.1?4.8 and 16.0?6.9, respectively. In the healthy controls, these percentages were 70.1?6.4, 3.5?4.6, 8.4?4.3 and 16.2?6.9, respectively. There were statistical significances between the 3 groups as to the above mentioned parameters (F values were 28.732, 3.867, 3.282 and 4.893, P<0.05 or P<0.01).The TZI and SDI were 1.306?0.123 and 1.057?0.180 in the AsAb positive patients, 1.255?0.096 and 0.901?0.119 in the control patients, and 1.257?0.098 and 0.882?0.121 in the healthy controls, respectively. There were statistical significances between the 3 groups (F values were 3.088 and 19.115, P<0.05 or P<0.01). Conclusions The positivity of AsAb is one of the reasons for the increasing of abnormal sperm morphology. Abnormal sperm morphology has mainly cone-shaped head, amorphous head and middle neck thickening sperms.

Key words: Anti-sperm antibody, Sperm morphology, Multiple sperm defect index

中图分类号:

R446.11

杨大干, 吕俊霞, 姚冬英, 沈乃仙. 抗精子抗体与精子形态的相关性调查[J]. 检验医学, 2013, 28(7): 590-594.

YANG Dagan,Li Junxia,YAO Dongying, SHEN Naixian. Correlation investigation of anti-sperm antibodies and sperm morphology[J]. , 2013, 28(7): 590-594.

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抗精子抗体与精子形态的相关性调查

Correlation investigation of anti-sperm antibodies and sperm morphology

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