检验医学 ›› 2013, Vol. 28 ›› Issue (2): 131-133.

• 临床应用研究.论著 • 上一篇    下一篇

不同脂血浓度对血液分析仪的影响

  

  1. 四川大学华西医院实验医学科临检血液室,四川 成都 610041
  • 出版日期:2013-02-28 发布日期:2013-01-28
  • 作者简介:曾素根,女,1961年生,主管技师,主要从事临床血液检验和教学工作。

Influence of different lipemia concentrations on automatic hematology analyzer

  1. Department of Blood,Department of Clinical Laboratory,Huaxi Hospital,Sichuan University,Sichuan Chengdu 610041,China
  • Online:2013-02-28 Published:2013-01-28

摘要: 目的 分析脂血对血液分析仪参数的干扰情况。 方法 正常新鲜全血标本在血液分析仪上进行全血细胞计数(CBC)后,将标本离心使血液中细胞与血浆分离,用相同体积的脂肪乳液置换出血浆,使血液中的甘油三脂(TG)>4.0 mmol/L,制成人工脂血标本,再用相同方法对脂血标本进行CBC分析,了解脂血对不同参数的干扰作用。 结果 当TG>4.0 mmol/L时,可使血红蛋白(Hb)、红细胞平均血红蛋白含量(MCH)和红细胞平均血红蛋白浓度(MCHC)等参数假性增高,差异有统计学意义(P<0.05);当血液中TG>14.45 mmol/L时,脂肪颗粒可使白细胞(WBC)计数假性增高,差异有统计学意义(P<0.05)。 结论 当血液中TG>4.0 mmol/L时,Hb、MCH和MCHC等参数需要进行纠正;TG浓度越高对Hb、MCH和MCHC的干扰越大;且Hb浓度越低受TG干扰程度越大。当血液中TG>14.45 mmol/L时,需要对WBC进行纠正。

关键词: 脂血干扰, 血液细胞分析

Abstract: Objective To investigate the interference of lipemia on automatic hematology analyzers.   Methods Normal fresh whole blood samples were determined for complete blood cell(CBC) count by automatic hematology analyzer,and the plasma were replaced by same volume of fat emulsion after centrifugal separation to make artificial lipemia [blood triglyceride(TG)>4.0 mmol/L]. These samples were tested for CBC by the same method before they were added lipid to estimate which parameters can be affected by lipemia.  Results When the TG>4.0 mmol/L,lipemia could make pseudo increase of hemoglobin(Hb),mean corpuscular hemoglobin(MCH) and mean corpuscular hemoglobin concentration(MCHC)(P<0.05). When the TG>14.45 mmol/L,fat particles can make pseudo increase of the white blood cell(WBC) count with statistical significance(P<0.05).  Conclusions When TG>4.0 mmol/L,Hb,MCH and MCHC need to be corrected. The higher TG concentration is,the greater interference of Hb,MCH and MCHC are. The lower Hb concentration is,the greater interference of TG is. When TG>14.45 mml/L,WBC needs to be corrected.

Key words: Lipemia interference, Blood cell analysis