CD40-1C/T基因多态性与儿童哮喘的相关性分析

检验医学 ›› 2013, Vol. 28 ›› Issue (1): 17-20.

CD40-1C/T基因多态性与儿童哮喘的相关性分析

1.南京医科大学附属苏州市立医院本部检验科，江苏苏州 215002；2. 南京医科大学附属苏州市立医院本部儿科，江苏苏州 215002

出版日期:2013-01-30 发布日期:2013-01-10
通讯作者: 徐红星，联系电话：0512-62362318。
作者简介:杜建钢，男，1977年生，主管技师，主要从事免疫检验工作。

Analysis on the correlation of CD40-1C/T gene polymorphism with children asthma

1.Department of Clinical Laboratory，Suzhou Municipal Hospital，Nanjing Medical University，Jiangsu Suzhou 215002，China；2.Department of Pediatrics，Suzhou Municipal Hospital，Nanjing Medical University，Jiangsu Suzhou 215002，China

Online:2013-01-30 Published:2013-01-10

摘要/Abstract

摘要：

目的　研究CD40基因5′非翻译区-1位点C/T多态性在本地区哮喘儿童人群中的分布，探讨其与哮喘发病风险之间的关系。方法　以134例哮喘患者作为研究对象，儿童门诊健康体检者113名作为对照组。应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测哮喘组和对照组CD40-1C/T位点的基因型及等位基因频率；采用免疫比浊法测定血清总IgE水平。分析该基因多态性与儿童哮喘发病风险及IgE水平之间的关系。结果　哮喘组和对照组之间C与T等位基因频率分布差异无统计学意义（P＞0.05)；CC基因型与CT+TT基因型之间的分布差异有统计学意义（P＜0.05)，CC基因型的发病风险是CT+TT基因型的1.75倍[95%可信区间（CI）：1.01～3.04]。哮喘组IgE水平在CC与CT、TT基因型间差异有统计学意义（P＜0.05）。结论　CD40基因5′非翻译区-1位点CC基因型可能与本地区儿童哮喘的发生有关。

关键词: CD40 , 哮喘, 基因多态性

Abstract:

Objective　To investigate the distribution of CD40 gene 5′untranslated region-1 site C/T gene polymorphism，and to study its relationship with the risk of asthma in local asthma children. Methods　A total of 134 asthma children were enrolled，and 113 healthy children from healthy examination subjects were as control group in asthma and contol groups. Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）was used to determine the genotype and the allele frequencies of CD40-1C/T gene. The serum total level of IgE was determined by immunonephelometry. The relationship of CD40-1C/T gene polymorphism with the risk of asthma and IgE level was analyzed. Results　The distributions of the C and T allele frequencies between asthma and control groups had no statistical significance （P＞0.05). The distribution difference of CC and CT+TT genotype frequencies was statistically significant （P＜0.05). The risk of CC genotype was 1.75 times higher than that of CT+TT genotype [95%confidence interval （CI）：1.01-3.04].There were significant differences in the level of IgE between CC genotype and CT or TT genotype in asthma group （P＜0.05). Conclusions　CC genotype of CD40 gene 5′untranslated region -1 site might be associated with the development of asthma in local asthma children.

Key words: CD40, Asthma, Gene polymorphism

杜建钢 1，徐红星 1，竭琼菲 1，钟桥 1，吴元建 1，徐吉成 2. CD40-1C/T基因多态性与儿童哮喘的相关性分析[J]. 检验医学, 2013, 28(1): 17-20.

DU Jiangang 1，XU Hongxing 1，JIE Qiongfei 1，ZHONG Qiao 1，WU Yuanjian 1，XU Jicheng 2. Analysis on the correlation of CD40-1C/T gene polymorphism with children asthma[J]. , 2013, 28(1): 17-20.

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