量子点标记快速免疫检测技术的研究

检验医学 ›› 2012, Vol. 27 ›› Issue (6): 448-450.

量子点标记快速免疫检测技术的研究

吴卫华

1.上海第九人民医院奉城分院，上海 201411；2.上海艾力斯医药科技有限公司，上海 201203

收稿日期:2012-02-07 修回日期:2012-02-27 出版日期:2012-06-30 发布日期:2012-06-06
通讯作者: 张鹏飞，联系电话：021-32502530。
作者简介:吴卫华，男，1963年生，主管技师，主要从事临床生化和临床免疫检验工作。

Studies on quantum dot labeling rapid immunoassay

1. Fengcheng Hospital，Shanghai Ninth People′s Hospital， Shanghai 201411，China；2.Shanghai Allist Pharmaceuticals Inc.，Shanghai 201203，China

Received:2012-02-07 Revised:2012-02-27 Online:2012-06-30 Published:2012-06-06

摘要/Abstract

摘要： 目的　自制发光材料量子点，优化量子点与抗体的偶联方案，并对制备的量子点标记抗体进行免疫检测的可行性研究。方法　采用高温热分解法制备得到镉硒（CdSe）量子点，用还原型谷胱甘肽（GSH）对量子点表面改性，使用双功能聚乙二醇（PEG）交联量子点表面的GSH，得到表面PEG化的水溶性量子点，该物质稳定性好。用抗乙型肝炎表面抗原（HBsAg）抗体与量子点共价交联，采用斑点免疫反应验证HBsAg检测的可行性。结果　成功制备量子点及其抗体标记物，检测HbsAg的敏感性可达1.6 ng/mL水平。结论　量子点标记免疫检测呈现技术优势，有广阔的发展与应用前景。

关键词: 量子点, 抗乙肝表面抗原抗体, 快速免疫检测

Abstract: Objective Based on prepared and modified quantum dots, conjugated with anti Hepatitis B antibodies under EDC activation to obtain quantum dot-antibody bioconjugates. To certify its usage in immune assay. Methods High temperature decomposition are used to prepare CdSe quantum dots, and modified with GSH and PEG separately for water solubility and stability. Conjugate quantum dots and goat-anti-HBsAg polyclone antibodies with ethyl-(dimethylaminopropyl) carbodiimide (EDC). Routine immune dot blot protocol was done for the test of HbsAg antigen. Results quantum dots were produced with uniformity and stability. The HBsAg antibody can be covalently linked with quantum dots and the bioconjugates were confirmed by various characterization. The bioconjugates can be used to detect 1.6 ng HBsAg proteins by dot blot immunoassay. Conclusion The new bioconjugates of conjugation of antibodies onto quantum dots for detection of the corresponding antigens provide better amplified signal immunoassays.

Key words: Quantum dot, Anti-hepatitis B surface antigen antibody, Rapid immunoassay

吴卫华;汤海英;张鹏飞. 量子点标记快速免疫检测技术的研究[J]. 检验医学, 2012, 27(6): 448-450.

WU Weihua;TANG Haiying;ZHANG Pengfei. Studies on quantum dot labeling rapid immunoassay[J]. , 2012, 27(6): 448-450.

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[2]	崔维韻;张建宁. 量子点在生物医学标记分析中的应用研究[J]. 检验医学, 2010, 25(02): 142-145.