缺氧诱导因子-2α在子宫颈鳞癌组织中的表达及临床意义

检验医学 ›› 2012, Vol. 27 ›› Issue (10): 819-821.

缺氧诱导因子-2α在子宫颈鳞癌组织中的表达及临床意义

江丽霞¹,施少华¹,施桥发²,李峰¹,刘四君¹

1. 赣南医学院第一附属医院，江西赣州 341000；2. 南昌大学，江西南昌 330000； 3. 赣南医学院，江西赣州 341000

收稿日期:2012-06-01 修回日期:2012-08-02 出版日期:2012-10-30 发布日期:2012-10-12
作者简介:江丽霞，女，1974年生，硕士，副主任技师，主要从事女性生殖系统肿瘤研究。
基金资助:
国家自然科学基金资助项目（30760280）；江西省自然科学基金资助项目（2011ZBAB204019）；江西省卫生厅课题资助项目（20072016，20113071）；赣南医学院重点课题资助项目（ZD2009005）

Expression and clinical significance of HIF-2α in cervical cancer tissues

1. First Affiliated Hospital of Gannan Medical College, Jiangxi Ganzhou 341000, China; 2. Nanchang University, Jiangxi Nanchang 330000, China; 3. Gannan Medical College, Jiangxi Ganzhou 341000, China

Received:2012-06-01 Revised:2012-08-02 Online:2012-10-30 Published:2012-10-12

摘要/Abstract

摘要： 目的　研究缺氧诱导因子-2α(HIF-2α)在子宫颈鳞癌组织中的表达情况及与肿瘤生物学特征的关系。方法　免疫组织化学染色方法检测子宫颈鳞癌组织中HIF-2α的表达。结果　40例子宫颈鳞癌组织中HIF-2α阳性表达得分为4.51±1.04，明显高于15例正常子宫颈组织的得分（2.28±1.60，P＜0.001）；子宫颈鳞癌患者中，有淋巴结转移的组织得分为5.00±0.89，也明显高于无淋巴结转移组织的得分（4.00±0.83，P＜0.01）；HIF-2α的阳性表达与子宫颈鳞癌患者的年龄无关，与子宫颈癌组织的FIGO2009分期、肿瘤大小有关。结论　HIF-2α的表达与子宫颈鳞癌的生长、侵袭有密切关系。

关键词: 缺氧诱导因子-2α, 子宫颈鳞癌, 免疫组织化学染色

Abstract: Objective　To investigate the relationship between hypoxia inducible factor-2 alpha (HIF-2α) expression and tumor biological characteristics in cervical cancer tissues. Methods　The expression of HIF-2α in cervical cancer tissues was detected by immunohistochemistry staining method. Results　The score of HIF-2α positive expression in 40 samples of cervical cancer tissues was 4.51±1.04. Compared with the score of 2.28±1.60 for 15 samples of normal cervical tissues, there was statistical significance between them (P＜0.001). In 40 samples of cervical cancer tissues, the score of HIF-2α positive expression in the samples with lymph node metastasis (5.00±0.89) was higher than that without lymph node metastasis (4.00±0.83, P<0.01). Although there was no relationship between the age of the patients and HIF-2α positive expression, the relationship of FIGO2009 staging and size of the tumor with HIF-2α positive expression was found. Conclusions　The expression of HIF-2α correlates closely with the growth and invasion of cervical cancer.

Key words: Hypoxia inducible factor-2 alpha, Cervical cancer, Immunohistochemistry staining

江丽霞1，施桥发2，施少华1，李峰1，刘四君3. 缺氧诱导因子-2α在子宫颈鳞癌组织中的表达及临床意义[J]. 检验医学, 2012, 27(10): 819-821.

JIANG Lixia 1，SHI Qiaofa 2，SHI Shaohua 1，LI Feng 1，LIU Sijun 3. Expression and clinical significance of HIF-2α in cervical cancer tissues[J]. , 2012, 27(10): 819-821.

参考文献

[1]丰有吉，沈铿. 妇产科学[M].第2版.北京：人民卫生出版社，2011：328.
[2]Bachtiary B, Schindl M, Ptter R, et al. Overexpression of hypoxia-inducible factor 1 alpha indicates diminished response to radiotherapy and unfavorable prognosis in patients receiving radical radiotherapy for cervical cancer[J]. Clin Cancer Res, 2003, 9 (6)：2234 -2240.
[3]Maynard MA, Ohh M. Von Hippel-Lindau tumor suppressor protein and hypoxia-inducible factor in kidney cancer[J]. Am J Nephrol, 2004,24(1)：1-13.
[4]Wan X, Shen N, Mendoza A, et al. CCI-779 inhibits rhabdomyosarcoma xenograft growth by an antiangiogenic mechanism linked to the targeting of mTOR/Hif-1alpha/VEGF signaling[J]. Neoplasia , 2006, 8(5)：394-401.
[5]Scortegagna M, Morris MA, Oktay Y, et al. The HIF family member EPAS1/HIF-2alpha is required for normal hematopoiesis in mice[J]. Blood, 2003, 102(5)：1634-1640.
[6]Bangoura G, Yang LY, Huang GW, et al. Expression of HIF-2 alpha/EPAS1 in hepatocellular carcinoma[J]. World J Gastroenterol, 2004, 10(4)：525-530.
[7]Uchida T, Rossignol F, Matthay MA，et al. Prolonged hypoxia differentially regulates hypoxia-inducible factor (HIF)-1 alpha and HIF-2 alpha expression in lung epithelial cells[J]. J Biol Chem, 2004, 279(15)：14871-14878.
[8]Wei L, Liu X, Hu C. Correlation between expression of HIF-2α and OCT-4 and prognosis of NSCLC[J]. Zhong Nan Da Xue Xue Bao Yi Xue Bao, 2011, 36(9)：854-858.
[9]Kim WY, Perera S, Zhou B, et al. HIF2 alpha co-operates with RAS to promote lung tumorigenesis in mice[J]. J Clin Invest, 2009, 119(8)：2160-2170.
[10]Li Z, Bao S, Wu Q, et al. Hypoxia-inducible factors regulate tumorigenic capacity of glioma stem cells[J]. Cancer Cell, 2009, 15(6)：501-513.
[11]〖JP+4〗Koukourakis MI, Giatromanolaki A, Sivridis E, et al. Hypoxia-inducible factor (HIF1A and HIF2A), angiogenesis, and chemoradiotherapy outcome of squamous cell head-and-neck cancer[J]. Int J Radiat Oncol Biol Phys, 2002,53(5)：1192-1202.
[12]Giatromanolaki A, Koukourakis MI, Sivridis E, et al. Relation of hypoxia inducible factor 1 alpha and 2 alpha in operable non-small cell lung cancer to angiogenic/molecular profile of tumours and survival[J]. Br J Cancer,2001,85(6)：881-890.