青蒿琥酯对痤疮丙酸杆菌的体外抑菌作用及机制研究

doi:10.3969/j.issn.1673-8640.2020.04.007

检验医学 ›› 2020, Vol. 35 ›› Issue (4): 318-322.DOI: 10.3969/j.issn.1673-8640.2020.04.007

青蒿琥酯对痤疮丙酸杆菌的体外抑菌作用及机制研究

陈向明, 何萍, 李婷, 张海清, 胡阳()

上海市嘉定区南翔医院皮肤科,上海 201802

收稿日期:2019-07-30 出版日期:2020-04-30 发布日期:2020-05-19
作者简介:null
作者简介：陈向明,女,1978年生,硕士,主管技师,主要从事皮肤性病实验研究。
基金资助:
上海市嘉定区农业和社会事业科研项目（JDKW-2016-W16）

In vitro antibacterial activity and mechanism of artesunate on Propionibacterium acnes

CHEN Xiangming, HE Ping, LI Tin, ZHANG Haiqing, HU Yang()

Department of Dermatology,Shanghai Jiading District Nanxiang Hospital,Shanghai 201802,China

Received:2019-07-30 Online:2020-04-30 Published:2020-05-19

摘要/Abstract

摘要：

目的探究青蒿琥酯对痤疮丙酸杆菌的体外抑菌作用及相关机制。方法采用微量肉汤稀释法检测痤疮丙酸杆菌标准株和临床株,观测青蒿琥酯联合盐酸多西环素的最小抑菌浓度（MIC）,并观察2种药物对痤疮丙酸杆菌生长的动态影响。采用扫描电镜观察青蒿琥酯作用后痤疮丙酸杆菌的形态学变化。结果青蒿琥酯MIC为0.750~3.000 mg/mL,盐酸多西环素MIC为0.040~0.160 μg/mL,两药联合使用呈相加或协同抑菌作用。两药在单药和联合使用时的MIC差异有统计学意义（P<0.05）。细菌生长动态观察结果显示两药联合使用可降低药物浓度,且抑菌作用起效快、持续时间长。扫描电镜观察结果显示青蒿琥酯作用后菌体变短,表面有皱瘪、凹陷、弯折等改变。结论青蒿琥酯对痤疮丙酸杆菌有一定抑菌作用,与盐酸多西环素联合使用抑菌效果优于单独使用,且对痤疮丙酸杆菌形态有影响。

关键词: 痤疮丙酸杆菌, 青蒿琥酯, 盐酸多西环素, 抑菌活性

Abstract:

Objective To investigate the in vitro antibacterial activity and mechanism of artesunate on Propionibacterium acnes. Methodse The minimum inhibitory concentrations（MIC） of artesunate combined with doxycycline hydrochloride to 14 standard and clinical isolates of Propionibacterium acnes were determined by broth microdilution method. Dynamic effects of the 2 drugs on the growth of Propionibacterium acnes were observed. Morphological changes of Propionibacterium acnes after artesunate treatment were observed by scanning electron microscopy. Results The MIC of artesunate was 0.750-3.000 mg/mL,and the MIC of doxycycline hydrochloride was 0.040-0.160 μg/mL. The 2 drugs showed additive or synergistic effect. The MIC difference between single drug and combined drugs was statistically significant（P<0.05）. Bacterial growth kinetics indicated that the combined drugs decreased the concentration of each drug,and the antibacterial action was rapid and long-lasting. Scanning electron microscopy showed that the bacteria became short after the action of artesunate,and the surface was wrinkled,sunk and curved. Conclusions Artesunate has antibacterial activity against Propionibacterium acnes. The combined action of artesunate and doxycycline hydrochloride is superior to single action. Artesunate has a morphological effect on Propionibacterium acnes.

Key words: Propionibacterium acnes, Artesunate, Doxycycline hydrochloride, Antibacterial activity

中图分类号:

R378.99

陈向明, 何萍, 李婷, 张海清, 胡阳. 青蒿琥酯对痤疮丙酸杆菌的体外抑菌作用及机制研究[J]. 检验医学, 2020, 35(4): 318-322.

CHEN Xiangming, HE Ping, LI Tin, ZHANG Haiqing, HU Yang. In vitro antibacterial activity and mechanism of artesunate on Propionibacterium acnes[J]. Laboratory Medicine, 2020, 35(4): 318-322.

图/表 4

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菌株	单独用药		联合用药
菌株	青蒿琥酯/（mg/mL）	盐酸多西环素/（µg/mL）	青蒿琥酯/（mg/mL）	盐酸多西环素/（µg/mL）	FIC
标准株	0.750	0.040	0.188	0.010	0.501
临床株 1	0.750	0.080	0.094	0.040	0.625
临床株 2	1.500	0.040	0.375	0.020	0.750
临床株 3	0.750	0.040	0.375	0.005	0.625
临床株 4	0.750	0.080	0.375	0.020	0.750
临床株 5	0.375	0.080	0.188	0.020	0.751
临床株 6	1.500	0.160	0.750	0.040	0.750
临床株 7	1.500	0.040	0.375	0.010	0.500
临床株 8	0.750	0.080	0.188	0.020	0.501
临床株 9	0.750	0.040	0.094	0.010	0.375
临床株 10	3.000	0.160	0.375	0.040	0.375
临床株 11	0.750	0.080	0.375	0.040	1.000
临床株 12	1.500	0.040	0.750	0.005	0.625
临床株 13	0.375	0.080	0.188	0.010	0.626

菌株	单独用药		联合用药
菌株	青蒿琥酯/（mg/mL）	盐酸多西环素/（µg/mL）	青蒿琥酯/（mg/mL）	盐酸多西环素/（µg/mL）	FIC
标准株	0.750	0.040	0.188	0.010	0.501
临床株 1	0.750	0.080	0.094	0.040	0.625
临床株 2	1.500	0.040	0.375	0.020	0.750
临床株 3	0.750	0.040	0.375	0.005	0.625
临床株 4	0.750	0.080	0.375	0.020	0.750
临床株 5	0.375	0.080	0.188	0.020	0.751
临床株 6	1.500	0.160	0.750	0.040	0.750
临床株 7	1.500	0.040	0.375	0.010	0.500
临床株 8	0.750	0.080	0.188	0.020	0.501
临床株 9	0.750	0.040	0.094	0.010	0.375
临床株 10	3.000	0.160	0.375	0.040	0.375
临床株 11	0.750	0.080	0.375	0.040	1.000
临床株 12	1.500	0.040	0.750	0.005	0.625
临床株 13	0.375	0.080	0.188	0.010	0.626

青蒿琥酯对痤疮丙酸杆菌的体外抑菌作用及机制研究

In vitro antibacterial activity and mechanism of artesunate on Propionibacterium acnes

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