关键词: 芯片检测技术, 阴道分泌物, 阴道炎, 病原学诊断

Abstract:

Objective To study the role of BD Affirm VPⅢ chip technology in determining vaginal secretions. Methods A total of 182 samples of vaginal secretions were determined by Nugent,trichomonad culturing,fungal culturing and BD Affirm VPⅢ,and the results of Nugent,trichomonad culturing,fungal culturing were used as gold standard. The results were analyzed statistically by SPSS 19. 0 software. Results Compared with Nugent,for bacterial vaginitis（BV）,the sensitivity of BD Affirm VPⅢ was 95.1%,the specificity was 83.8%,and Kappa value was 0.80. When the results of fungal culturing were used as gold standard,for determining Candida,the sensitivity of BD Affirm VPⅢ was 75.8%,the specificity was 99.0%,and Kappa value was 0.85. When the results of trichomonad culturing were used as gold standard,for determining trichomonad,the sensitivity of BD Affirm VPⅢ was 92.9%,the specificity was 100.0%,and Kappavalue was 0.96. Conclusions BD Affirm VPⅢ chip technology is convenient and can be simultaneously determine 3 kinds of pathogenic microorganisms for vaginitis,and it has high sensitivity and specificity. It overcomes the limitation of traditional determination methods with clinical application significance.

Key words: Chip technology, Vaginal secretion, Vaginitis, Etiological diagnosis