检验医学 ›› 2017, Vol. 32 ›› Issue (10): 867-870.DOI: 10.3969/j.issn.1673-8640.2017.010.007

• 临床应用研究·论著 • 上一篇    下一篇

IL-26在复发性自然流产患者蜕膜中的表达

梁昀, 施幼豪, 李明清, 段忠亮   

  1. 复旦大学附属妇产科医院,上海 200011
  • 收稿日期:2017-02-22 出版日期:2017-10-20 发布日期:2017-11-20
  • 作者简介:null

    作者简介:梁 昀,女,1975年生,技师,主要从事临床检验工作。

    通信作者:段忠亮,联系电话:021-33189900-8286。

  • 基金资助:
    国家自然科学基金面上项目(31671200);上海市卫生和计划生育委员会青年科研项目(20164Y0105)

Expressions of IL-26 in decidua from recurrent spontaneous abortion patients

LIANG Yun, SHI Youhao, LI Mingqing, DUAN Zhongliang   

  1. Obstetrics and Gynecology Hospital of Fudan University,Shanghai 200011,China
  • Received:2017-02-22 Online:2017-10-20 Published:2017-11-20

摘要:

目的 探讨白细胞介素26(IL-26)在复发性自然流产(RSA)患者蜕膜中的调节作用。方法 用免疫组织化学方法检测IL-26及其受体IL-10R2和IL-20R1在15例正常早孕者和18例RSA患者蜕膜组织中的表达情况,用酶联免疫吸附试验(ELISA)检测2组间蜕膜细胞分泌IL-26的情况,用不同浓度(1、10和100 ng/mL)的IL-26刺激正常早孕者的蜕膜细胞,于24、48和72 h后检测细胞的增殖能力。结果 IL-26及其受体在RSA组蜕膜组织的表达水平高于早孕组(P<0.05)。RSA组蜕膜细胞分泌IL-26的水平高于早孕组(P<0.05)。用1和10 ng/mL的IL-26刺激有利于蜕膜细胞增殖,但100 ng/mL的IL-26会抑制蜕膜细胞的增殖。结论 IL-26在蜕膜组织中的过高表达不利于蜕膜细胞的增殖,可能与RSA的发生相关。

关键词: 白细胞介素26, 复发性自然流产, 蜕膜

Abstract:

Objective To evaluate the role of interleukin 26(IL-26) in decidua from recurrent spontaneous abortion(RSA) patients. Methods The expressions of IL-26 and its receptors(IL-10R2 and IL-20R1) were determined by immunohistochemistry in 15 healthy pregnant women and 18 RSA patients. The secretion status of IL-26 in decidual stromal cells between the 2 groups was determined by enzyme-linked immunosorbent assay(ELISA). The decidual stromal cells of healthy pregnant women were stimulated with recombinant human IL-26 protein(1,10 and 100 ng/mL),and then cell proliferation was determined after 24,48 and 72 h. Results The expressions of IL-26,IL-10R2 and IL-20R1 in RSA patients' decidual tissues were higher than those in healthy pregnant women(P<0.05). The level of IL-26 secreted by decidual stromal cells in RSA patients was higher than that in healthy pregnant women(P<0.05). The proliferation of decidual stromal cells was promoted by IL-26 at the levels of 1 and 10 ng/mL,but it was inhibited at the level of 100 ng/mL. Conclusions The overexpression of IL-26 may restrain the proliferation of decidual stromal cells and be associated with RSA.

Key words: Interleukin 26, Recurrent spontaneous abortion, Decidua

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