复发性自然流产患者T细胞中Tim-3和PD-1的表达

doi:10.3969/j.issn.1673-8640.2016.06.008

检验医学 ›› 2016, Vol. 31 ›› Issue (6): 474-478.DOI: 10.3969/j.issn.1673-8640.2016.06.008

复发性自然流产患者T细胞中Tim-3和PD-1的表达

段忠亮, 施幼豪, 李明清, 李翠

复旦大学附属妇产科医院,上海 200011

收稿日期:2016-02-01 出版日期:2016-06-30 发布日期:2016-07-05
作者简介:null
作者简介：段忠亮,男,1986年生,学士,技师,主要从事临床免疫学检验工作。

通讯作者：李翠,联系电话：021-33189900-8372。
基金资助:
国家自然科学基金资助项目（31101064）;上海市卫生系统新优青计划资助项目（XYQ2013104）;复旦大学卓学计划资助项目

Expressions of Tim-3 and PD-1 on T cells in patients with recurrent spontaneous abortion

DUAN Zhongliang, SHI Youhao, LI Mingqing, LI Cui

Obstetrics and Gynecology Hospital of Fudan University,Shanghai 200011,China

Received:2016-02-01 Online:2016-06-30 Published:2016-07-05

摘要/Abstract

摘要：

目的探讨复发性自然流产（RSA）患者T细胞免疫球蛋白黏蛋白-3（Tim-3）及程序性死亡因子-1（PD-1）在T细胞中的表达情况,为其作为治疗RSA的潜在靶点提供理论依据。方法采集22名正常早孕妇女（对照组）及21例RSA患者抗凝外周血,用流式细胞术分别检测T细胞表面分子Tim-3及PD-1的表达情况。结果 RSA患者外周血T细胞中Tim-3⁺T细胞比例（1.01%±0.64%）和PD-1⁺T细胞比例（40.76%±13.76%）均明显低于对照组（1.85%±0.96%、54.32%±26.65%）（P<0.01、P<0.05）;RSA组T细胞中Tim-3⁺PD-1⁺双阳性T细胞比例（0.59%±0.27%）明显低于对照组（1.37%±0.85%,P<0.001）,而Tim-3^-PD-1⁺T细胞比例（40.17%±13.68%）和Tim-3⁺PD-1^-T细胞比例（0.43%±0.41%）与对照组（52.95%±26.28%、0.48%±0.31%）比较,差异无统计学意义（P>0.05）。结论 Tim-3⁺CD3⁺T细胞、PD-1⁺CD3⁺T细胞及Tim-3⁺PD-1⁺双阳性T细胞水平降低可能与RSA有关,有作为RSA治疗潜在靶点的价值。

关键词: T细胞免疫球蛋白黏蛋白-3, 程序性死亡因子-1, 复发性自然流产

Abstract:

Objective To investigate the expressions of T cell immunoglobin mucin-3 （Tim-3） and programmed death-1 （PD-1） on T cells in patients with recurrent spontaneous abortion（RSA）,and provide a reference for target treatment. Method The expressions of Tim-3 and PD-1 on T cells were determined by flow cytometry in 22 healthy pregnant women （control group） and 21 patients with RSA. Result The proportion of Tim-3⁺ T cells in RSA group （1.01%±0.64%） was lower than that in control group （1.85%±0.96%）（P<0.01）,and the proportion of PD-1⁺ T cells in RSA group（40.76%±13.76%） was lower than that in control group （54.32%±26.65%）（P<0.05）. The proportion of Tim-3⁺ PD-1⁺ T cells in RSA group（0.59%±0.27%） was lower than that in control group（1.37%±0.85%）（P<0.001）,while the proportions of Tim-3^-PD-1⁺ and Tim-3⁺ PD-1^-T cells（40.17%±13.68% and 0.43%±0.41%） had no statistical significance with those in control group（52.95%±26.28% and 0.48%±0.31%）（P>0.05）. ConclusionsThe expressions of Tim-3⁺CD3⁺,PD-1⁺CD3⁺ and Tim-3⁺ PD-1⁺ T cells are correlated with RSA. They can be potential targets for RSA treatment.

Key words: T cell immunoglobin mucin-3, Programmed death-1, Recurrent spontaneous abortion

中图分类号:

R446.1

段忠亮, 施幼豪, 李明清, 李翠. 复发性自然流产患者T细胞中Tim-3和PD-1的表达[J]. 检验医学, 2016, 31(6): 474-478.

DUAN Zhongliang, SHI Youhao, LI Mingqing, LI Cui. Expressions of Tim-3 and PD-1 on T cells in patients with recurrent spontaneous abortion[J]. Laboratory Medicine, 2016, 31(6): 474-478.

图/表 2

参考文献 25

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复发性自然流产患者T细胞中Tim-3和PD-1的表达

Expressions of Tim-3 and PD-1 on T cells in patients with recurrent spontaneous abortion

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