实时荧光RT-PCR在人感染H7N9禽流感病毒检测中的应用

doi:10.3969/j.issn.1673-8640.2013.09.005

检验医学 ›› 2013, Vol. 28 ›› Issue (9): 755-757.DOI: 10.3969/j.issn.1673-8640.2013.09.005

• H7N9禽流感的实验室检测专题 • 上一篇下一篇

实时荧光RT-PCR在人感染H7N9禽流感病毒检测中的应用

华哲云,褚维,宋黎黎,王宇,高淑娜,劳宝法

上海市黄浦区疾病预防控制中心,上海 200023

收稿日期:2013-06-14 修回日期:2013-09-25 出版日期:2013-09-15 发布日期:2013-09-25
通讯作者: 劳宝法,联系电话:021-63013388。
作者简介:华哲云,女,1970年生,主管技师,主要从事病原微生物实验室检测和研究。

Application of real-time fluorescence RT-PCR to detect avian influenza A(H7N9) virus

HUA Zheyun, CHU Wei, SONG Lili, WANG Yu, GAO Shuna, LAO Baofa.

Huangpu Center for Disease Control and Prevention,Shanghai 200023, China

Received:2013-06-14 Revised:2013-09-25 Online:2013-09-15 Published:2013-09-25

摘要/Abstract

摘要：

目的评估实时荧光逆转录聚合酶链反应(RT-PCR)检测H7N9禽流感病毒的应用效果,为人感染H7N9禽流感监测和防控提供可靠的实验室检测数据。方法采集各级医疗单位人感染H7N9禽流感监测及可疑病例标本137例,同时使用4种实时荧光RT-PCR试剂分别检测上呼吸道标本和下呼吸道标本中H7N9禽流感病毒核酸。结果 H7N9禽流感病毒核酸阳性率为3.65%(5/137);H7N9禽流感病毒核酸阳性标本中上呼吸道标本检出率为60%(3/5),下呼吸道标本检出率为100%(3/3)。4种试剂符合率为100%。结论实时荧光RT-PCR能有效检出H7N9禽流感病毒核酸,检测下呼吸道标本有助于提高阳性检出率。

关键词: H7N9禽流感病毒, 人类感染, 实时荧光逆转录聚合酶链反应, 下呼吸道标本

Abstract:

Objective To assess the application effect of real-time fluorescence reverse transcription-polymerase chain reaction (RT-PCR) to detect avian influenza A(H7N9) virus, and to provide reliable data to laboratory detection for avian influenza A(H7N9) virus surveillance, prevention and control. Methods A total of 137 being monitored and suspected specimens from hospitals were collected, and the nucleic acids of avian influenza A(H7N9)virus in specimens from upper and lower respiratory tracts were detected by 4 kinds of real-time fluorescence RT-PCR kits. Results The positive rate of avian influenza A(H7N9)virus was 3.65%(5/137). Among the positive specimens,the positive rate of upper respiratory tract was 60%(3/5), and the positive rate of lower respiratory tract was 100%(3/3). The coincidence rate of 4 kinds of kits was 100%. Conclusions Real-time fluorescence RT-PCR can detect the nucleic acid of avian influenza A(H7N9)virus effectively. Specimens from lower respiratory tract contribute to improve the positive detection rate of avian influenza A (H7N9) virus.

Key words: Avian influenza A(H7N9) virus, Human infection, Real-time fluorescence reverse transcription-polymerase chain reaction, Lower respiratory tract specimen

中图分类号:

R373.1

华哲云,褚维,宋黎黎,王宇,高淑娜,劳宝法. 实时荧光RT-PCR在人感染H7N9禽流感病毒检测中的应用[J]. 检验医学, 2013, 28(9): 755-757.

HUA Zheyun, CHU Wei, SONG Lili, WANG Yu, GAO Shuna, LAO Baofa.. Application of real-time fluorescence RT-PCR to detect avian influenza A(H7N9) virus[J]. , 2013, 28(9): 755-757.

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实时荧光RT-PCR在人感染H7N9禽流感病毒检测中的应用

Application of real-time fluorescence RT-PCR to detect avian influenza A(H7N9) virus

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