• 生物化学检验论著 •

### 基于健康人群唾液葡萄糖检测体系的构建

1. 1. 暨南大学生物医学工程研究所，广州 广东 510632；2. Biomedical Information Technology Laboratory，University of Aizu，Aizu-Wakamatsu，Fukushima 965-8580，Japan
• 出版日期:2012-06-30 发布日期:2012-06-06
• 作者简介:罗　曼，女，1963年生，博士，教授，主要研究方向为生物化学与分子生物学及生物物理。

### Establishment of saliva glucose detection system for healthy subjects

1. 1. Institute of Biomedical Engineering，Ji′nan University，Guangdong Guangzhou 510632，China；2. Biomedical Information Technology Laboratory， University of Aizu， Fukushima Aizu-Wakamatsu 965-8580，Japan
• Online:2012-06-30 Published:2012-06-06

Abstract: Objective　To establish a high sensitive detection system to detect the glucose level in saliva in order to realize the daily noninvasively blood glucose monitoring for healthy subjects.  Methods　The method of glucose oxidase-peroxidase with 2，4，6-tribromo-3-hydroxybenzoic acid（TBHBA） as chromogen was modified， in which pH value and the consistency of ion were optimized， and citral was added to adjust the activity of the enzyme.  Results　The reaction of the new detection system could be completed in 30 min， and the maximal absorbing wavelength of detecting absorbance（A） value was 510 nm. The optimization of the new detection system parameters was pH: 5.8，0.3 mol/L NaCl and 1 220 μg/mL citral. The low limit value of glucose level detection was 0.006 mmol/L. Conclusions　The sensitivity of the optimized detection system is about 7 times more than that of the original system. The low limit value is 1/10 of fasting saliva glucose mean level in healthy subjects. It is sensitive enough to detect the glucose level in saliva of healthy subjects.