Laboratory Medicine ›› 2024, Vol. 39 ›› Issue (6): 592-597.DOI: 10.3969/j.issn.1673-8640.2024.06.014
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Received:
2023-08-22
Revised:
2023-10-20
Online:
2024-06-30
Published:
2024-07-08
CLC Number:
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URL: https://www.shjyyx.com/EN/10.3969/j.issn.1673-8640.2024.06.014
靶基因 | 引物序列(5'→3') | 目标片段长度/bp |
---|---|---|
spa[ | 1113F:TAAAGACGATCCTTCGGTGAGC | 244~ 465 |
1514R:CAGCAGTAGTGCCGTTTGCTT | ||
pvl[ | F:GTGCCAGACAATGAATTACCC | 256 |
R:TTCATGAGTTTTCCAGTTCACTTC | ||
psm- mec[ | F:GAAGATCTATCACAAGATGAAATA | 210 |
R:ATGGATTTCACTGGTGTTATTACA | ||
mecA[ | F:GTGAAGATATACCAAGTGATT | 147 |
R:ATGCGCTATAGATTGAAAGGAT | ||
qacA/B[ | F:GTTGGAGCTGGTATGGCTTCAC | 352 |
R:TGATAATCCCACCTACTAAAGC |
靶基因 | 引物序列(5'→3') | 目标片段长度/bp |
---|---|---|
spa[ | 1113F:TAAAGACGATCCTTCGGTGAGC | 244~ 465 |
1514R:CAGCAGTAGTGCCGTTTGCTT | ||
pvl[ | F:GTGCCAGACAATGAATTACCC | 256 |
R:TTCATGAGTTTTCCAGTTCACTTC | ||
psm- mec[ | F:GAAGATCTATCACAAGATGAAATA | 210 |
R:ATGGATTTCACTGGTGTTATTACA | ||
mecA[ | F:GTGAAGATATACCAAGTGATT | 147 |
R:ATGCGCTATAGATTGAAAGGAT | ||
qacA/B[ | F:GTTGGAGCTGGTATGGCTTCAC | 352 |
R:TGATAATCCCACCTACTAAAGC |
spa类型 | 株数 | mecA+ | psm-mec+ | pvl+ | qacA/B+ |
---|---|---|---|---|---|
t309 | 5 | 未检出 | 未检出 | 5 | 1 |
t034 | 4 | 4 | 未检出 | 未检出 | 未检出 |
t164 | 2 | 未检出 | 未检出 | 未检出 | 1 |
t002 | 2 | 1 | 2 | 1 | 1 |
t441 | 1 | 1 | 未检出 | 未检出 | 未检出 |
t437 | 1 | 1 | 未检出 | 未检出 | 未检出 |
t3622 | 1 | 1 | 未检出 | 未检出 | 未检出 |
t16634 | 1 | 1 | 未检出 | 未检出 | 未检出 |
t1255 | 1 | 1 | 未检出 | 未检出 | 未检出 |
t5635 | 1 | 1 | 未检出 | 未检出 | 未检出 |
t127 | 1 | 未检出 | 未检出 | 未检出 | 1 |
t5763 | 1 | 未检出 | 未检出 | 1 | 未检出 |
合计 | 21 | 11 | 2 | 7 | 4 |
spa类型 | 株数 | mecA+ | psm-mec+ | pvl+ | qacA/B+ |
---|---|---|---|---|---|
t309 | 5 | 未检出 | 未检出 | 5 | 1 |
t034 | 4 | 4 | 未检出 | 未检出 | 未检出 |
t164 | 2 | 未检出 | 未检出 | 未检出 | 1 |
t002 | 2 | 1 | 2 | 1 | 1 |
t441 | 1 | 1 | 未检出 | 未检出 | 未检出 |
t437 | 1 | 1 | 未检出 | 未检出 | 未检出 |
t3622 | 1 | 1 | 未检出 | 未检出 | 未检出 |
t16634 | 1 | 1 | 未检出 | 未检出 | 未检出 |
t1255 | 1 | 1 | 未检出 | 未检出 | 未检出 |
t5635 | 1 | 1 | 未检出 | 未检出 | 未检出 |
t127 | 1 | 未检出 | 未检出 | 未检出 | 1 |
t5763 | 1 | 未检出 | 未检出 | 1 | 未检出 |
合计 | 21 | 11 | 2 | 7 | 4 |
spa分型 | SCCmec分型 | ST分型 | 管家基因 | ||||||
---|---|---|---|---|---|---|---|---|---|
gmk | tpi | glpF | pta | arcC | yqiL | aroE | |||
t437 | Ⅳg(2B) | ST59 | 2 | 20 | 15 | 19 | 19 | 15 | 23 |
t441 | Ⅳa(2B) | ST59 | 2 | 20 | 15 | 19 | 19 | 15 | 23 |
t002 | Ⅱ(2A) | ST764 | 4 | 1 | 1 | 12 | 1 | 10 | 136 |
t034 | Ⅴ(5C2) | ST398 | 2 | 26 | 19 | 20 | 3 | 39 | 35 |
t034 | Ⅴ(5C2) | ST398 | 2 | 26 | 19 | 20 | 3 | 39 | 35 |
t034 | Ⅴ(5C2) | ST398 | 2 | 26 | 19 | 20 | 3 | 39 | 35 |
t16634 | Ⅳa(2B) | ST6547 | 176 | 20 | 15 | 19 | 19 | 15 | 23 |
t1255 | Ⅴ(5C2) | ST398 | 2 | 26 | 19 | 20 | 3 | 39 | 35 |
t034 | Ⅴ(5C2) | ST398 | 2 | 26 | 19 | 20 | 3 | 39 | 35 |
t5635 | Ⅴ(5C2) | ST398 | 2 | 26 | 19 | 20 | 3 | 39 | 35 |
t3622 | Ⅳc(2B) | ST88 | 23 | 4 | 14 | 12 | 22 | 31 | 1 |
spa分型 | SCCmec分型 | ST分型 | 管家基因 | ||||||
---|---|---|---|---|---|---|---|---|---|
gmk | tpi | glpF | pta | arcC | yqiL | aroE | |||
t437 | Ⅳg(2B) | ST59 | 2 | 20 | 15 | 19 | 19 | 15 | 23 |
t441 | Ⅳa(2B) | ST59 | 2 | 20 | 15 | 19 | 19 | 15 | 23 |
t002 | Ⅱ(2A) | ST764 | 4 | 1 | 1 | 12 | 1 | 10 | 136 |
t034 | Ⅴ(5C2) | ST398 | 2 | 26 | 19 | 20 | 3 | 39 | 35 |
t034 | Ⅴ(5C2) | ST398 | 2 | 26 | 19 | 20 | 3 | 39 | 35 |
t034 | Ⅴ(5C2) | ST398 | 2 | 26 | 19 | 20 | 3 | 39 | 35 |
t16634 | Ⅳa(2B) | ST6547 | 176 | 20 | 15 | 19 | 19 | 15 | 23 |
t1255 | Ⅴ(5C2) | ST398 | 2 | 26 | 19 | 20 | 3 | 39 | 35 |
t034 | Ⅴ(5C2) | ST398 | 2 | 26 | 19 | 20 | 3 | 39 | 35 |
t5635 | Ⅴ(5C2) | ST398 | 2 | 26 | 19 | 20 | 3 | 39 | 35 |
t3622 | Ⅳc(2B) | ST88 | 23 | 4 | 14 | 12 | 22 | 31 | 1 |
[1] | LEE A S, DE LENCASTRE H, GARAU J, et al. Methicillin-resistant Staphylococcus aureus[J]. Nat Rev Dis Primers, 2018, 4:18033. |
[2] | BODE L G, KLUYTMANS J A, WERTHEIM H F, et al. Preventing surgical-site infections in nasal carriers of Staphylococcus aureus[J]. N Engl J Med, 2010, 362(1):9-17. |
[3] | WERTHEIM H F, MELLES D C, VOS M C, et al. The role of nasal carriage in Staphylococcus aureus infections[J]. Lancet Infect Dis, 2005, 5(12):751-762. |
[4] | AL AMIRY A, BISSELL R A, MAGUIRE B J, et al. Methicillin-resistant Staphylococcus aureus nasal colonization prevalence among Emergency Medical Services personnel[J]. Prehosp Disaster Med, 2013, 28(4):348-352. |
[5] | Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing[S]. M100- ed31,CLSI, 2021. |
[6] | MOHAMMED K A S, ABDULKAREEM Z H, ALZAALAN A R, et al. Spa typing of Staphylococcus aureus isolated from clinical specimens from outpatients in Iraq[J]. Pol J Microbiol, 2021, 70(1):79-85. |
[7] | 刘根焰, 黄一宁, 赵旺胜, 等. 金黄色葡萄球菌sasX、psm-mec、pvl三种毒力基因的检测与分析[J]. 临床检验杂志, 2013, 31(10):744-746. |
[8] | ZHANG K, MCCLURE J A, ELSAYED S, et al. Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types Ⅰ to Ⅴ in methicillin-resistant Staphylococcus aureus[J]. J Clin Microbiol, 2005, 43(10):5026-5033. |
[9] | 郭震, 张燕莲, 陈传, 等. 主动外排泵系统qacA/B、杀白细胞素与耐甲氧西林金黄色葡萄球菌耐药性的研究[J]. 中国卫生检验杂志, 2014, 24(23):3484-3487. |
[10] | KLUYTMANS J, VAN BELKUM A, VERBRUGH H. Nasal carriage of Staphylococcus aureus:epidemiology,underlying mechanisms,and associated risks[J]. Clin Microbiol Rev, 1997, 10(3):505-520. |
[11] | SAUD B, KHATRI G, AMATYA N, et al. Methicillin-resistant and biofilm-producing Staphylococcus aureus in nasal carriage among health care workers and medical students[J]. Can J Infect Dis Med Microbiol, 2023, 2023:8424486. |
[12] | STEFANI S, CHUNG D R, LINDSAY J A, et al. Meticillin-resistant Staphylococcus aureus(MRSA):global epidemiology and harmonisation of typing methods[J]. Int J Antimicrob Agents, 2012, 39(4):273-282. |
[13] | LIM K L, KHOR W C, ONG K H, et al. Occurrence and patterns of enterotoxin genes,spa types and antimicrobial resistance patterns in Staphylococcus aureus in food and food contact surfaces in Singapore[J]. Microorganisms, 2023, 11(7):1785. |
[14] | ILCZYSZYN W M, SABAT A J, AKKERBOOM V, et al. Clonal structure and characterization of Staphylococcus aureus strains from invasive infections in paediatric patients from south Poland:association between age,spa types,clonal complexes,and genetic markers[J]. PLoS One, 2016, 11(3):e0151937. |
[15] | MIKO B A, HAFER C A, LEE C J, et al. Molecular characterization of methicillin-susceptible Staphylococcus aureus clinical isolates in the United States,2004 to 2010[J]. J Clin Microbiol, 2013, 51(3):874-879. |
[16] | SAFFARI F, RADFAR A, SOBHANIPOOR M H, et al. Spa gene-based molecular typing of nasal methicillin-susceptible Staphylococcus aureus from patients and health-care workers in a dialysis center in southeast Iran[J]. Pathog Glob Health, 2020, 114(3):160-163. |
[17] | VERKOLA M, TAKALA M, NYKÄSENOJA S, et al. Low-level colonization of methicillin-resistant Staphylococcus aureus in pigs is maintained by slowly evolving,closely related strains in finnish pig farms[J]. Acta Vet Scand, 2022, 64(1):34. |
[18] | KITTLER S, SEINIGE D, MEEMKEN D, et al. Characteristics of methicillin-resistant Staphylococcus aureus from broiler farms in Germany are rather lineage- than source-specific[J]. Poult Sci, 2019, 98(12):6903-6913. |
[19] | WITTE W, STROMMENGER B, STANEK C, et al. Methicillin-resistant Staphylococcus aureus ST398 in humans and animals,Central Europe[J]. Emerg Infect Dis, 2007, 13(2):255-258. |
[20] | CHEN Y, LIU Z, DUO L, et al. Characterization of Staphylococcus aureus from distinct geographic locations in China:an increasing prevalence of spa-t030 and SCCmec type Ⅲ[J]. PLoS One, 2014, 9(4):e96255. |
[21] | XU Z, LI X, TIAN D, et al. Molecular characterization of methicillin-resistant and -susceptible Staphylococcus aureus recovered from hospital personnel[J]. J Med Microbiol, 2020, 69(12):1332-1338. |
[22] | KINNEVEY P M, KEARNEY A, SHORE A C, et al. Meticillin-susceptible Staphylococcus aureus transmission among healthcare workers,patients and the environment in a large acute hospital under non-outbreak conditions investigated using whole-genome sequencing[J]. J Hosp Infect, 2022, 127:15-25. |
[23] | YAMAGUCHI T, ONO D, SATO A. Staphylococcal cassette chromosome mec(SCCmec)analysis of MRSA[J]. Methods Mol Biol, 2020, 2069:59-78. |
[24] | ARMAND-LEFEVRE L, RUIMY R, ANDREMONT A. Clonal comparison of Staphylococcus aureus isolates from healthy pig farmers,human controls,and pigs[J]. Emerg Infect Dis, 2005, 11(5):711-714. |
[25] | CUI M, LI J, ALI T, et al. Emergence of livestock-associated MRSA ST398 from bulk tank milk,China[J]. J Antimicrob Chemother, 2020, 75(12):3471-3474. |
[26] | PARISI A, CARUSO M, NORMANNO G, et al. High occurrence of methicillin-resistant Staphylococcus aureus in horses at slaughterhouses compared with those for recreational activities:a professional and food safety concern?[J]. Foodborne Pathog Dis, 2017, 14(12):735-741. |
[27] | KAITO C, SAITO Y, NAGANO G, et al. Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence[J]. PLoS Pathog, 2011, 7(2):e1001267. |
[28] | BOUNDY S, ZHAO Q, FAIRBANKS C, et al. Spontaneous staphylococcal cassette chromosome mec element excision in Staphylococcus aureus nasal carriers[J]. J Clin Microbiol, 2012, 50(2):469-471. |
[29] | CHI C Y, LIN C C, LIAO I C, et al. Panton-valentine leukocidin facilitates the escape of Staphylococcus aureus from human keratinocyte endosomes and induces apoptosis[J]. J Infect Dis, 2014, 209(2):224-235. |
[30] | SIMOR A E. Staphylococcal decolonisation:an effective strategy for prevention of infection?[J]. Lancet Infect Dis, 2011, 11(12):952-962. |
[31] | BATRA R, COOPER B S, WHITELEY C, et al. Efficacy and limitation of a chlorhexidine-based decolonization strategy in preventing transmission of methicillin-resistant Staphylococcus aureus in an intensive care unit[J]. Clin Infect Dis, 2010, 50(2):210-217. |
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