Abstract:

Objective To study the development and determination quality of fungal culture identification and in vitro drug sensitivity test in Shanghai through external quality assessment（EQA）. Methods In 2019,EQA issued a total of 10 samples for culture and identification,of which 4 samples required further drug sensitivity test. According to the feedback results,the coincidence rate of fungal identification and the development of in vitro drug sensitivity test were analyzed. Results A total of 75 effective returns were received,45 of which were tested for fungal drug sensitivity. The 4 of 10 strains（including 2 genera and 9 species） were identified with 100% coincidence rate,and the strains with wrong identification results were Candida lusitaniae,Candida guilliermondii,Candida parapsilosis,Candida albicans,Cryptococcus laurentii and Cryptococcus neoformans. In the first EQA in 2019,93.3%（70/75） of the laboratory results were completely correct,6.7%（5/75） of the laboratories had wrong results,but the total score was qualified. In the second EQA,94.7%（71/75） of the laboratory results were completely correct,4.0%（3/75） of the laboratories had wrong results,but the total scores were qualified,and 1.3%（1/75） of the laboratories were unqualified. Conclusions The overall determination ability and accuracy rate of fungal culture and identification in clinical laboratories in Shanghai are relatively high. The number of clinical laboratories carrying out fungal drug sensitivity test is limited. Quality control in clinical laboratories is essential to assure the accuracy of results.

Key words: Fungus, Culture identification, In vitro drug sensitivity test, External quality assessment, Quality control