Drug resistance mechanism of carbapenem resistant Providencia rettgeri carrying blaNDM-1

doi:10.3969/j.issn.1673-8640.2019.01.014

Abstract

Abstract:

Objective To investigate the phenotype,mechanism,disseminative way and homology of carbapenem resistant Providencia rettgeri. Methods A total of 17 isolates of Providencia rettgeri isolated from intensive care unit （ICU） were collected,and antibiotic susceptibility test,drug resistance gene detection,NP test,pulsed-field gel electrophoresis （PFGE）,plasmid mobility analysis and replicon typing were performed. Results The 17 isolates of carbapenem resistant Providencia rettgeri carried bla_NDM-1. The drug resistance rates to ertapenem,meropenem,imipenem,cefazolin,cefuroxime,cefotaxime,cefepime,cefmetazole,piperacillin,trimethoprim-sulfamethoxazole,amikacin,gentamicin,tobramycin,kanamycin,ciprofloxacin and furadantin were 100.00%. The drug resistance rates to piperacillin-tazobactam and aztreonam were 88.23%. PFGE homology analysis showed that 15 isolates were the main epidemic isolates in ICU. Plasmid analysis showed that the bla_NDM-1 were located on conjugative plasmid （-160 kb）,and replicon type was type A/C. Plasmid carried a variety of drug resistance genes,being sensitive to ciprofloxacin and furadantin,and it mediated the high-level drug resistance to carbapenems,cephalosporins,cephamycins,sulfonamides and aminoglycosides. Conclusions Providencia rettgeri carrying bla_NDM-1 shows multi-drug resistance to common antibiotics,and it brings challenge to clinical anti-infection treatment.

Key words: Providencia rettgeri, Carbapenem resistance, bla_NDM-1gene, Drug resistance mechanism, Intensive care unit

CLC Number:

R378.99

LI Wei, AI Fuqi, MA Yimin, WANG Bei, TIAN Yueru. Drug resistance mechanism of carbapenem resistant Providencia rettgeri carrying bla_NDM-1[J]. Laboratory Medicine, 2019, 34(1): 67-70.

Figures/Tables 1

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