Abstract:

Objective To study the influence of indoor carbon dioxide（CO₂） concentration on the stability of CO₂ reagents by enzymatic method. Methods A total of 2 CO₂ reagents by enzymatic method were used and treated by 2 ways. Reagent 1 was sealed at 4 ℃ in refrigerator after daily work finished. Reagent 2 was continuously left in instrument reagent positions and exposed to air until the end of experiment,which was simulating normal working state. The 2 reagents were used to determine the CO₂ levels in 3 samples of high-value quality control material,low-value quality control material and mixed serum at different time points,and indoor CO₂ concentration was determined to observe the change of the determination results of 2 reagents by enzymatic method with indoor CO₂ concentration changing. Pearson correlation analysis was used to analyze the correlation between the determination results of 3 samples and indoor CO₂concentration. Results The CO₂ levels of 3 samples determined by the 2 reagents showed the same trend as indoor CO₂ concentration with the increasing of reagent loading time. Correlation analysis showed that the determination results of 3 samples were correlated with indoor CO₂ concentration（Reagent 1：r=0.722,0.853 and 0.790,P<0.01;Reagent 2：0.689,0.875 and 0.801,P<0.01）. There was no correlation between indoor CO₂ concentration and reagent loading time（Reagent 1：r=-0.132,-0.108 and -0.103,P>0.05;Reagent 2：-0.124,-0.112 and -0.111,P>0.05）. Conclusions The stability of CO₂ reagents by enzymatic method is influenced by indoor CO₂ concentration. The accuracy of CO₂ reagents by enzymatic method can be ensured by using sealed reagent,reducing population density or increasing ventilation frequency.

Key words: Carbon dioxide, Serum, Stability