Influence of AJUBA overexpression on T47D gene expression profile by RNA sequencing

doi:10.3969/j.issn.1673-8640.2016.06.018

Abstract

Abstract:

Objective To investigate the influence of AJUBA gene on estrogen receptor-positive breast cancer cell line T47D gene expression. Methods T47D cell line for stable expression of AJUBA was established. RNA-seq analysis was performed for RNA extracted from T47D-vector and T47D-AJUBA groups. After mapping and reading,transcriptome reconstruction,normalization and expression quantification,differentially expressed genes were identified with gene ontology（GO） analysis and Kyoto Encyclopedia of Genes and Genomes（KEGG） pathway enrichment analysis. Differentially expressed genes were validated by real-time fluorescence quantitative polymerase chain reaction（RT-qPCR）. Results A total of 568 differentially expressed genes were identified,including 239 up-regulated genes and 329 down-regulated genes. In GO analysis,3,23 and 8 up-regulated genes were annotated to molecular function,biological process and cellular component,respectively,while 21,35 and 9 down-regulated genes were also annotated to molecular function,biological process and cellular component,respectively. In KEGG analysis,2 were up-regulated genes,and 4 were down-regulated genes. Conclusions The overexpression of AJUBA gene may have remarkable effects on a series of biological function-related multiple signal pathways,and AJUBA gene might play an important role in the tumorigenesis and development of breast cancer.

Key words: AJUBA, Breast cancer, High-throughput RNA sequencing, Differentially expressed gene, Bioinformatics analysis

CLC Number:

R446.1

XU Beihui, LI Qi, ZOU Xiuqun, XU Hong, HOU Zhaoyuan, WANG Jiamin, NI Peihua. Influence of AJUBA overexpression on T47D gene expression profile by RNA sequencing[J]. Laboratory Medicine, 2016, 31(6): 527-532.

Figures/Tables 4

References 17

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作用	途径	基因数	占总基因百分比（%）	P值
上调	PPAR 信号通路	4	2.515 723 27	0.012 399 522
	ErbB 信号通路	4	2.515 723 27	0.023 003 129
下调	轴突导向	7	2.857 142 857	0.005 757 691
	VEGF信号通路	5	2.040 816 327	0.015 001 19
	肥厚性心肌病	5	2.040 816 327	0.022 738 371
	扩张性心肌病	5	2.040 816 327	0.029 377 142

作用	途径	基因数	占总基因百分比（%）	P值
上调	PPAR 信号通路	4	2.515 723 27	0.012 399 522
	ErbB 信号通路	4	2.515 723 27	0.023 003 129
下调	轴突导向	7	2.857 142 857	0.005 757 691
	VEGF信号通路	5	2.040 816 327	0.015 001 19
	肥厚性心肌病	5	2.040 816 327	0.022 738 371
	扩张性心肌病	5	2.040 816 327	0.029 377 142