Clinical application of serum folate and red blood cell folate determinations

doi:10.3969/j.issn.1673-8640.2016.03.019

Abstract

Abstract:

Folate deficiency is closely related to neural tube defect in newborns and megaloblastic anemia. The determinations of serum folate and red blood cell folate could be primary evidence for folate deficiency. However,the determination methods have not yet been standardized. This article will describe the biological characteristics of folate,the causes of folate deficiency,its epidemiological study,clinical manifestation,diagnostic cut-off values and the clinical application of folate determinations.

Key words: Folate, Megaloblastic anemia, Neural tube defect

CLC Number:

R446.1

CHEN Pu, PAN Baishen. Clinical application of serum folate and red blood cell folate determinations[J]. Laboratory Medicine, 2016, 31(3): 232-236.

Figures/Tables 3

References 11

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项目	红细胞叶酸	血清叶酸
个体叶酸储量	长期水平	短期水平
样本类型	全血	血清或血浆
分析前影响因素
饮食影响	不受影响	受近期摄入影响
稳定性和储存条件	全血比红细胞溶液更稳定;4℃保存4 d 内的存留率>90%;22 ℃保存4 d内的存留率>80%;冻融对结果有很大影响	4 ℃保存1周内的存留率<10%;血清比血浆更稳定;对冻融敏感,但对3次以内冻融影响不大（每次冻融放置时间为1 h）
样本准备	需人工进行血细胞溶血	无
生物学变异^*
个体内（％）	32.1	3.20
个体间（％）	36.4	48.90
分析中影响因素^#
方法精密度（％）	3.9~6.0	5.4~12.0
方法间差异（％）	3.3~66.5	1.8~11.1
分析后影响因素
临床吻合度	非常好（有5%的大红细胞症患者血清叶酸正常）	好
费用	由于人工成本高,花费的费用比血清叶酸稍高	–
注：*数据来源于NHANES/Westgard生物学变异数据库;#数据来源于美国病理学家协会（the College of American Pathologists,CAP）的临床治疗（physical therapy,PT）调查报告

项目	红细胞叶酸	血清叶酸
个体叶酸储量	长期水平	短期水平
样本类型	全血	血清或血浆
分析前影响因素
饮食影响	不受影响	受近期摄入影响
稳定性和储存条件	全血比红细胞溶液更稳定;4℃保存4 d 内的存留率>90%;22 ℃保存4 d内的存留率>80%;冻融对结果有很大影响	4 ℃保存1周内的存留率<10%;血清比血浆更稳定;对冻融敏感,但对3次以内冻融影响不大（每次冻融放置时间为1 h）
样本准备	需人工进行血细胞溶血	无
生物学变异^*
个体内（％）	32.1	3.20
个体间（％）	36.4	48.90
分析中影响因素^#
方法精密度（％）	3.9~6.0	5.4~12.0
方法间差异（％）	3.3~66.5	1.8~11.1
分析后影响因素
临床吻合度	非常好（有5%的大红细胞症患者血清叶酸正常）	好
费用	由于人工成本高,花费的费用比血清叶酸稍高	–
注：*数据来源于NHANES/Westgard生物学变异数据库;#数据来源于美国病理学家协会（the College of American Pathologists,CAP）的临床治疗（physical therapy,PT）调查报告

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