Evaluation of 2 platelet estimation methods using peripheral blood smear

doi:10.3969/j.issn.1673-8640.2015.10.015

Abstract

Abstract: Objective

To evaluate the clinical significance of 2 platelet estimation methods using peripheral blood smear in thrombocytopenia patients.

Methods

A total of 227 ethylene diamine tetraacetic acid-K₂ (EDTA-K₂) anticoagulant blood samples were collected and performed by Sysmex XE-2100 automatic hematology analyzer. The blood smears were completed and stained by Sysmex SP1000i automatic blood smear instrument.

Method

A and B were used to estimate platelet count. The results of 2 platelet estimation methods were compared, and the correlations and Bland-Altman consistency analysis were performed.

Results

Platelet counts from

Method

A and B showed correlation coefficients of 0.894 and 0.867 when compared with automatic hematology analyzer (P<0.000 1). Bland-Altman analysis showed that 2 methods and automatic hematology analyzer had no systematic bias, and it had consistency for platelet count.

Conclusions

Blood smear platelet estimation shows good correlation and consistency with automatic hematology analyzer. It is useful for the verification of the low platelet results of automatic hematology analyzer in clinical practice.

Key words: Peripheral blood smear, Platelet estimation count, Automatic hematology analyzer

CLC Number:

R446.11

ZHU Jianfeng, ZHANG Li, WANG Beili, GUO Wei, PAN Baishen.. Evaluation of 2 platelet estimation methods using peripheral blood smear[J]. Laboratory Medicine, 2015, 30(10): 1027-1029.

Figures/Tables 4

References 9

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